View source for 1k2w

[[Image:1k2w.jpg|left|200px]]

 {{Structure
|PDB= 1k2w |SIZE=350|CAPTION= <scene name='initialview01'>1k2w</scene>, resolution 2.4&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/L-iditol_2-dehydrogenase L-iditol 2-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.14 1.1.1.14] </span>
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k2w OCA], [http://www.ebi.ac.uk/pdbsum/1k2w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k2w RCSB]</span>
}}

'''Crystal structure of sorbitol dehydrogenase from R. sphaeroides'''


==Overview==
Recombinant sorbitol dehydrogenase (SDH) from Rhodobacter sphaeroides has been crystallized in the absence of the cofactor NAD(H) and its structure determined to 2.4 A resolution using molecular replacement (refined R and R free factors of 18.8 and 23.8%, respectively). As expected from the sequence and shown by the conserved fold, SDH can be assigned to the short-chain dehydrogenase/reductase protein family. The cofactor NAD and the substrate sorbitol have been modelled into the structure and the active-site architecture, which displays the highly conserved catalytic tetrad of Asn-Ser-Tyr-Lys residues, is discussed in relation to the enzyme mechanism. This is the first structure of a bacterial SDH belonging to the SDR family.

==About this Structure==
1K2W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2W OCA]. 

==Reference==
Structure of zinc-independent sorbitol dehydrogenase from Rhodobacter sphaeroides at 2.4 A resolution., Philippsen A, Schirmer T, Stein MA, Giffhorn F, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):374-9. Epub 2005, Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805591 15805591]
[[Category: L-iditol 2-dehydrogenase]]
[[Category: Rhodobacter sphaeroides]]
[[Category: Single protein]]
[[Category: Philippsen, A.]]
[[Category: Schirmer, T.]]
[[Category: Stetefeld, J.]]
[[Category: enzyme]]
[[Category: short-chain dehydrogenase]]

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