View source for 1jfa

[[Image:1jfa.jpg|left|200px]]

 {{Structure
|PDB= 1jfa |SIZE=350|CAPTION= <scene name='initialview01'>1jfa</scene>, resolution 2.5&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trichodiene_synthase Trichodiene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.6 4.2.3.6] </span>
|GENE= TRI5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 Fusarium sporotrichioides])
|DOMAIN=
|RELATEDENTRY=[[1jfg|1JFG]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jfa OCA], [http://www.ebi.ac.uk/pdbsum/1jfa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jfa RCSB]</span>
}}

'''TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES'''


==Overview==
The x-ray crystal structure of recombinant trichodiene synthase from Fusarium sporotrichioides has been determined to 2.5-A resolution, both unliganded and complexed with inorganic pyrophosphate. This reaction product coordinates to three Mg(2+) ions near the mouth of the active site cleft. A comparison of the liganded and unliganded structures reveals a ligand-induced conformational change that closes the mouth of the active site cleft. Binding of the substrate farnesyl diphosphate similarly may trigger this conformational change, which would facilitate catalysis by protecting reactive carbocationic intermediates in the cyclization cascade. Trichodiene synthase also shares significant structural similarity with other sesquiterpene synthases despite a lack of significant sequence identity. This similarity indicates divergence from a common ancestor early in the evolution of terpene biosynthesis.

==About this Structure==
1JFA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFA OCA]. 

==Reference==
Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade., Rynkiewicz MJ, Cane DE, Christianson DW, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11698643 11698643]
[[Category: Fusarium sporotrichioides]]
[[Category: Single protein]]
[[Category: Trichodiene synthase]]
[[Category: Cane, D E.]]
[[Category: Christianson, D W.]]
[[Category: Rynkiewicz, M J.]]
[[Category: terpenoid synthase fold]]

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