View source for 1irz

[[Image:1irz.gif|left|200px]]

 {{Structure
|PDB= 1irz |SIZE=350|CAPTION= <scene name='initialview01'>1irz</scene>
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1irz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1irz OCA], [http://www.ebi.ac.uk/pdbsum/1irz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1irz RCSB]</span>
}}

'''Solution structure of ARR10-B belonging to the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators'''


==Overview==
The B motif is a signature of type-B response regulators (ARRs) involved in His-to-Asp phosphorelay signal transduction systems in Arabidopsis. Homologous motifs occur widely in the GARP family of plant transcription factors. To gain general insight into the structure and function of B motifs (or GARP motifs), we characterized the B motif derived from a representative ARR, ARR10, which led to a number of intriguing findings. First, the B motif of ARR10 (named ARR10-B and extending from Thr-179 to Ser-242) possesses a nuclear localization signal, as indicated by the intracellular localization of a green fluorescent protein-ARR10-B fusion protein in onion epidermal cells. Second, the purified ARR10-B molecule binds specifically in vitro to DNA with the core sequence AGATT. This was demonstrated by several in vitro approaches, including PCR-assisted DNA binding site selection, gel retardation assays, and surface plasmon resonance analysis. Finally, the three-dimensional structure of ARR10-B in solution was determined by NMR spectroscopy, showing that it contains a helix-turn-helix structure. Furthermore, the mode of interaction between ARR10-B and the target DNA was assessed extensively by NMR spectroscopy. Together, these results lead us to propose that the mechanism of DNA recognition by ARR10-B is essentially the same as that of homeodomains. We conclude that the B motif is a multifunctional domain responsible for both nuclear localization and DNA binding and suggest that these insights could be applicable generally to the large GARP family of plant transcription factors.

==About this Structure==
1IRZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRZ OCA]. 

==Reference==
Molecular structure of the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators., Hosoda K, Imamura A, Katoh E, Hatta T, Tachiki M, Yamada H, Mizuno T, Yamazaki T, Plant Cell. 2002 Sep;14(9):2015-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12215502 12215502]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Hosoda, K.]]
[[Category: Katoh, E.]]
[[Category: Mizuno, T.]]
[[Category: Yamazaki, T.]]
[[Category: helix-turn-helix]]

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