View source for 2ckp

[[Image:2ckp.gif|left|200px]]<br />
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'''CRYSTAL STRUCTURE OF HUMAN CHOLINE KINASE ALPHA-2 IN COMPLEX WITH ADP'''<br />

==Overview==
Choline kinase, responsible for the phosphorylation of choline to, phosphocholine as the first step of the CDP-choline pathway for the, biosynthesis of phosphatidylcholine, has been recognized as a new target, for anticancer therapy. Crystal structures of human choline kinase in its, apo, ADP and phosphocholine-bound complexes, respectively, reveal the, molecular details of the substrate binding sites. ATP binds in a cavity, where residues from both the N and C-terminal lobes contribute to form a, cleft, while the choline-binding site constitutes a deep hydrophobic, groove in the C-terminal domain with a rim composed of negatively charged, residues. Upon binding of choline, the enzyme undergoes conformational, changes independently affecting the N-terminal domain and the ATP-binding, loop. From this structural analysis and comparison with other kinases, and, from mutagenesis data on the homologous Caenorhabditis elegans choline, kinase, a model of the ternary ADP.phosphocholine complex was built that, reveals the molecular basis for the phosphoryl transfer activity of this, enzyme.

==About this Structure==
2CKP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Choline_kinase Choline kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.32 2.7.1.32] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CKP OCA]. 

==Reference==
Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine., Malito E, Sekulic N, Too WC, Konrad M, Lavie A, J Mol Biol. 2006 Nov 24;364(2):136-51. Epub 2006 Sep 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17007874 17007874]
[[Category: Choline kinase]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Lavie, A.]]
[[Category: Malito, E.]]
[[Category: ADP]]
[[Category: alternative splicing]]
[[Category: choline kinase]]
[[Category: kinase]]
[[Category: phosphatydilcholine]]
[[Category: transferase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 15:35:41 2007''