View source for 1vzy

[[Image:1vzy.gif|left|200px]]<br />
<applet load="1vzy" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1vzy, resolution 1.97&Aring;" />
'''CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS HSP33'''<br />

==Overview==
The bacterial heat shock protein Hsp33 is a redox-regulated chaperone, activated by oxidative stress. In response to oxidation, four cysteines, within a Zn2+ binding C-terminal domain form two disulfide bonds with, concomitant release of the metal. This leads to the formation of the, biologically active Hsp33 dimer. The crystal structure of the N-terminal, domain of the E. coli protein has been reported, but neither the structure, of the Zn2+ binding motif nor the nature of its regulatory interaction, with the rest of the protein are known. Here we report the crystal, structure of the full-length B. subtilis Hsp33 in the reduced form. The, structure of the N-terminal, dimerization domain is similar to that of the, E. coli protein, although there is no domain swapping. The Zn2+ binding, domain is clearly resolved showing the details of the tetrahedral, coordination of Zn2+ by four thiolates. We propose a structure-based, activation pathway for Hsp33.

==About this Structure==
1VZY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with ZN and ACT as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: ZNB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VZY OCA]. 

==Reference==
The crystal structure of the reduced, Zn2+-bound form of the B. subtilis Hsp33 chaperone and its implications for the activation mechanism., Janda I, Devedjiev Y, Derewenda U, Dauter Z, Bielnicki J, Cooper DR, Graf PC, Joachimiak A, Jakob U, Derewenda ZS, Structure. 2004 Oct;12(10):1901-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15458638 15458638]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Bielnicki, J.]]
[[Category: Cooper, D.R.]]
[[Category: Dauter, Z.]]
[[Category: Derewenda, U.]]
[[Category: Derewenda, Z.S.]]
[[Category: Devedjiev, Y.]]
[[Category: Janda, I.K.]]
[[Category: Joachimiak, A.]]
[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]
[[Category: ACT]]
[[Category: ZN]]
[[Category: crystal engineering]]
[[Category: heat shock protein]]
[[Category: mcsg]]
[[Category: midwest center for structural genomics]]
[[Category: molecular chaperone]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: redox-active center]]

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