View source for 1eg0

[[Image:1eg0.gif|left|200px]]

 {{Structure
|PDB= 1eg0 |SIZE=350|CAPTION= <scene name='initialview01'>1eg0</scene>, resolution 11.5&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=4SU:4-THIOURIDINE-5&#39;-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5&#39;-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=N:ANY+5&#39;-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=OMC:O2&#39;-METHYLYCYTIDINE-5&#39;-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=T:THYMIDINE-5&#39;-MONOPHOSPHATE'>T</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg0 OCA], [http://www.ebi.ac.uk/pdbsum/1eg0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eg0 RCSB]</span>
}}

'''FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME'''


==Overview==
Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.

==About this Structure==
1EG0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG0 OCA]. 

==Reference==
Solution structure of the E. coli 70S ribosome at 11.5 A resolution., Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P, Cell. 2000 Mar 3;100(5):537-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10721991 10721991]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Agrawal, R K.]]
[[Category: Frank, J.]]
[[Category: Gabashvili, I S.]]
[[Category: Grassucci, R A.]]
[[Category: Penczek, P.]]
[[Category: Spahn, C M.T.]]
[[Category: Svergun, D I.]]
[[Category: 70s ribosome]]
[[Category: low resolution model]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:01:48 2008''