View source for 1e9r

[[Image:1e9r.jpg|left|200px]]

 {{Structure
|PDB= 1e9r |SIZE=350|CAPTION= <scene name='initialview01'>1e9r</scene>, resolution 2.40&Aring;
|SITE= <scene name='pdbsite=SO1:So4+Binding+Site+For+Residue+G602+Nucleotide-Binding+Sit+...'>SO1</scene>
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
|ACTIVITY= 
|GENE= TRWB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e9r OCA], [http://www.ebi.ac.uk/pdbsum/1e9r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e9r RCSB]</span>
}}

'''BACTERIAL CONJUGATIVE COUPLING PROTEIN TRWBDELTAN70. TRIGONAL FORM IN COMPLEX WITH SULPHATE.'''


==Overview==
The transfer of DNA across membranes and between cells is a central biological process; however, its molecular mechanism remains unknown. In prokaryotes, trans-membrane passage by bacterial conjugation, is the main route for horizontal gene transfer. It is the means for rapid acquisition of new genetic information, including antibiotic resistance by pathogens. Trans-kingdom gene transfer from bacteria to plants or fungi and even bacterial sporulation are special cases of conjugation. An integral membrane DNA-binding protein, called TrwB in the Escherichia coli R388 conjugative system, is essential for the conjugation process. This large multimeric protein is responsible for recruiting the relaxosome DNA-protein complex, and participates in the transfer of a single DNA strand during cell mating. Here we report the three-dimensional structure of a soluble variant of TrwB. The molecule consists of two domains: a nucleotide-binding domain of alpha/beta topology, reminiscent of RecA and DNA ring helicases, and an all-alpha domain. Six equivalent protein monomers associate to form an almost spherical quaternary structure that is strikingly similar to F1-ATPase. A central channel, 20 A in width, traverses the hexamer.

==About this Structure==
1E9R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E9R OCA]. 

==Reference==
The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase., Gomis-Ruth FX, Moncalian G, Perez-Luque R, Gonzalez A, Cabezon E, de la Cruz F, Coll M, Nature. 2001 Feb 1;409(6820):637-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11214325 11214325]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Cabezon, E.]]
[[Category: Coll, M.]]
[[Category: Cruz, F De La.]]
[[Category: Gomis-Rueth, F X.]]
[[Category: Moncalian, G.]]
[[Category: bacterial conjugation]]
[[Category: coupling protein]]
[[Category: f1-atpase-like quaternary structure]]
[[Category: ring helicase]]

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