View source for 1dvo

[[Image:1dvo.jpg|left|200px]]

 {{Structure
|PDB= 1dvo |SIZE=350|CAPTION= <scene name='initialview01'>1dvo</scene>, resolution 2.00&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
|DOMAIN=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dvo OCA], [http://www.ebi.ac.uk/pdbsum/1dvo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dvo RCSB]</span>
}}

'''THE X-RAY CRYSTAL STRUCTURE OF FINO, A REPRESSOR OF BACTERIAL CONJUGATION'''


==Overview==
The conjugative transfer of F-like plasmids is repressed by FinO, an RNA binding protein. FinO interacts with the F-plasmid encoded traJ mRNA and its antisense RNA, FinP, stabilizing FinP against endonucleolytic degradation and facilitating sense-antisense RNA recognition. Here we present the 2.0 A resolution X-ray crystal structure of FinO, lacking its flexible N-terminal extension. FinO adopts a novel, elongated, largely helical conformation. An N-terminal region, previously shown to contact RNA, forms a positively charged alpha-helix (helix 1) that protrudes 45 A from the central core of FinO. A C-terminal region of FinO that is implicated in RNA interactions also extends out from the central body of the protein, adopting a helical conformation and packing against the base of the N-terminal helix. A highly positively charged patch on the surface of the FinO core may present another RNA binding surface. The results of an in vitro RNA duplexing assay demonstrate that the flexible N-terminal region of FinO plays a key role in FinP-traJ RNA recognition, and supports our proposal that this region and the N-terminus of helix 1 interact with and stabilize paired, complementary RNA loops in a kissing complex.

==About this Structure==
1DVO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVO OCA]. 

==Reference==
Crystal structure of the bacterial conjugation repressor finO., Ghetu AF, Gubbins MJ, Frost LS, Glover JN, Nat Struct Biol. 2000 Jul;7(7):565-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10876242 10876242]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Frost, L S.]]
[[Category: Ghetu, A F.]]
[[Category: Glover, J N.M.]]
[[Category: Gubbins, M J.]]
[[Category: bacterial conjugation]]
[[Category: repressor]]
[[Category: x-ray crystallography]]

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