View source for 2c1w

==THE STRUCTURE OF XENDOU: A SPLICING INDEPENDENT SNORNA PROCESSING ENDORIBONUCLEASE==
<StructureSection load='2c1w' size='340' side='right' caption='[[2c1w]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2c1w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C1W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2c09|2c09]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c1w OCA], [http://pdbe.org/2c1w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c1w RCSB], [http://www.ebi.ac.uk/pdbsum/2c1w PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/ENDUA_XENLA ENDUA_XENLA]] Poly(U)-specific endoribonuclease involved in the biosynthesis of intron-encoded box C/D snoRNAs, such as U16 and U86. Releases products that have 2'-3'-cyclic phosphate termini.<ref>PMID:12571235</ref> <ref>PMID:15755742</ref> <ref>PMID:7513048</ref> <ref>PMID:9144568</ref>  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c1/2c1w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c1w ConSurf].
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== Publication Abstract from PubMed ==
Small nucleolar RNAs (snoRNAs) play a key role in eukaryotic ribosome biogenesis. In most cases, snoRNAs are encoded in introns and are released through the splicing reaction. Some snoRNAs are, instead, produced by an alternative pathway consisting of endonucleolytic processing of pre-mRNA. XendoU, the endoribonuclease responsible for this activity, is a U-specific, metal-dependent enzyme that releases products with 2'-3' cyclic phosphate termini. XendoU is broadly conserved among eukaryotes, and it is a genetic marker of nidoviruses, including the severe acute respiratory syndrome coronavirus, where it is essential for replication and transcription. We have determined by crystallography the structure of XendoU that, by refined search methodologies, appears to display a unique fold. Based on sequence conservation, mutagenesis, and docking simulations, we have identified the active site. The conserved structural determinants of this site may provide a framework for attempting to design antiviral drugs to interfere with the infectious nidovirus life cycle.

The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication.,Renzi F, Caffarelli E, Laneve P, Bozzoni I, Brunori M, Vallone B Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12365-70. Epub 2006 Aug 8. PMID:16895992<ref>PMID:16895992</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2c1w" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: African clawed frog]]
[[Category: Bozzoni, I]]
[[Category: Brunori, M]]
[[Category: Caffarelli, E]]
[[Category: Laneve, P]]
[[Category: Renzi, F]]
[[Category: Vallone, B]]
[[Category: Endoribonuclease]]
[[Category: Nuclease]]
[[Category: Snorna]]
[[Category: Splicing independent processing]]