View source for 158d

[[Image:158d.gif|left|200px]]

 {{Structure
|PDB= 158d |SIZE=350|CAPTION= <scene name='initialview01'>158d</scene>, resolution 1.900&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
|ACTIVITY= 
|GENE= 
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=158d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=158d OCA], [http://www.ebi.ac.uk/pdbsum/158d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=158d RCSB]</span>
}}

'''CRYSTALLOGRAPHIC ANALYSIS OF C-C-A-A-G-C-T-T-G-G AND ITS IMPLICATIONS FOR BENDING IN B-DNA'''


==Overview==
Stacked B-DNA double helices of sequence C-C-A-A-G-C-T-T-G-G exhibit the same 23 degrees bend at -T-G-G C-C-A- across the nonbonded junction between helices that is observed in the middle of the decamer helix of sequence C-A-T-G-G-C-C-A-T-G, even though the space group (hexagonal vs orthorhombic), crystal packing, and connectedness at the center of the bent segment are quite different. An identical bend occurs across the interhelix junction of every monoclinic crystal structure of sequence C-C-A-x-x-x-x-T-G-G, suggesting that T-G-G-C-C-A constitutes a natural bending element in B-DNA. The bend occurs by rolling stacked base pairs about their long axes; there is no "tilt" component. Of the three possible models for A-tract bending--bent-A-tract, junction bends, or bent-non-A--which cannot be distinguished by solution measurements, all crystallographic evidence over the past 10 years unanimously supports the non-A regions as the actual bending loci.

==About this Structure==
158D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=158D OCA]. 

==Reference==
Crystallographic analysis of C-C-A-A-G-C-T-T-G-G and its implications for bending in B-DNA., Grzeskowiak K, Goodsell DS, Kaczor-Grzeskowiak M, Cascio D, Dickerson RE, Biochemistry. 1993 Aug 31;32(34):8923-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8364037 8364037]
[[Category: Protein complex]]
[[Category: Cascio, D.]]
[[Category: Dickerson, R E.]]
[[Category: Goodsell, D S.]]
[[Category: Grzeskowiak, K.]]
[[Category: Kaczor-Grzeskowiak, M.]]
[[Category: b-dna]]
[[Category: double helix]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:28:33 2008''