View source for 2c9k

==STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION==
<StructureSection load='2c9k' size='340' side='right' caption='[[2c9k]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2c9k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C9K FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c9k OCA], [http://pdbe.org/2c9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c9k RCSB], [http://www.ebi.ac.uk/pdbsum/2c9k PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CR4AA_BACTI CR4AA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects. 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/2c9k_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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== Publication Abstract from PubMed ==
The Cry4Aa delta-endotoxin from Bacillus thuringiensis is toxic to larvae of Culex, Anopheles, and Aedes mosquitoes, which are vectors of important human tropical diseases. With the objective of designing modified toxins with improved potency that could be used as biopesticides, we determined the structure of this toxin in its functional form at a resolution of 2.8 angstroms. Like other Cry delta-endotoxins, the activated Cry4Aa toxin consists of three globular domains, a seven-alpha-helix bundle responsible for pore formation (domain I) and the following two other domains having structural similarities with carbohydrate binding proteins: a beta-prism (domain II) and a plant lectin-like beta-sandwich (domain III). We also studied the effect on toxicity of amino acid substitutions and deletions in three loops located at the surface of the putative receptor binding domain II of Cry4Aa. Our results indicate that one loop is an important determinant of toxicity, presumably through attachment of Cry4Aa to the surface of mosquito cells. The availability of the Cry4Aa structure should guide further investigations aimed at the molecular basis of the target specificity and membrane insertion of Cry endotoxins.

Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution.,Boonserm P, Mo M, Angsuthanasombat C, Lescar J J Bacteriol. 2006 May;188(9):3391-401. PMID:16621834<ref>PMID:16621834</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2c9k" style="background-color:#fffaf0;"></div>

==See Also==
*[[Delta-endotoxin|Delta-endotoxin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]
[[Category: Angsuthanasombat, C]]
[[Category: Boonserm, P]]
[[Category: Lescar, J]]
[[Category: Mo, M]]
[[Category: Bioinsecticicide]]
[[Category: Insect toxin]]
[[Category: Pore formation]]
[[Category: Receptor recognition]]
[[Category: Sporulation]]
[[Category: Toxin]]