View source for 1m3s

[[Image:1m3s.jpg|left|200px]]

 {{Structure
|PDB= 1m3s |SIZE=350|CAPTION= <scene name='initialview01'>1m3s</scene>, resolution 1.95&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= YckF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0794 GutQ]</span>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m3s OCA], [http://www.ebi.ac.uk/pdbsum/1m3s PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1m3s RCSB]</span>
}}

'''Crystal structure of YckF from Bacillus subtilis'''


==Overview==
The crystal structure of the YckF protein from Bacillus subtilis was determined with MAD phasing and refined at 1.95A resolution. YckF forms a tight tetramer both in crystals and in solution. Conservation of such oligomerization in other phosphate sugar isomerases indicates that the crystallographically observed tetramer is physiologically relevant. The structure of YckF was compared to with its ortholog from Methanococcus jannaschii, MJ1247. Both of these proteins have phosphate hexulose isomerase activity, although neither of the organisms can utilize methane or methanol as source of energy and/or carbon. Extensive sequence and structural similarities with MJ1247 and with the isomerase domain of glucosamine-6-phosphate synthase from Escherichia coli allowed us to group residues contributing to substrate binding or catalysis. Few notable differences among these structures suggest possible cooperativity of the four active sites of the tetramer. Phylogenetic relationships between obligatory and facultative methylotrophs along with B. subtilis and E. coli provide clues about the possible evolution of genes as they loose their physiological importance.

==About this Structure==
1M3S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M3S OCA]. 

==Reference==
Crystal structure of Bacillus subtilis YckF: structural and functional evolution., Sanishvili R, Wu R, Kim DE, Watson JD, Collart F, Joachimiak A, J Struct Biol. 2004 Oct;148(1):98-109. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15363790 15363790]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Collart, F.]]
[[Category: Joachimiak, A.]]
[[Category: Kim, D E.]]
[[Category: MCSG, Midwest Center for Structural Genomics.]]
[[Category: Sanishvili, R.]]
[[Category: Wu, R.]]
[[Category: mcsg]]
[[Category: midwest center for structural genomic]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomic]]

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