View source for 2uvu

[[Image:2uvu.jpg|left|200px]]

 {{Structure
|PDB= 2uvu |SIZE=350|CAPTION= <scene name='initialview01'>2uvu</scene>, resolution 2.70&Aring;
|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+A'>AC1</scene>
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=DGT:2&#39;-DEOXYGUANOSINE-5&#39;-TRIPHOSPHATE'>DGT</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] 
|GENE= 
}}

'''CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS'''


==Overview==
Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly efficient and relatively accurate manner. Crystal structures have revealed a potential role for Arg(332) in stabilizing the anti conformation of the 8-oxoG template base by means of a hydrogen bond or ion-dipole pair, which results in an increased enzymatic efficiency for dCTP insertion and makes formation of a Hoogsteen pair between 8-oxoG and dATP less favorable. Site-directed mutagenesis was used to replace Arg(332) with Ala, Glu, Leu, or His in order to probe the importance of Arg(332) in accurate and efficient bypass of 8-oxoG. The double mutant Ala(331)Ala(332) was also prepared to address the contribution of Arg(331). Transientstate kinetic results suggest that Glu(332) retains fidelity against bypass of 8-oxoG that is similar to wild type Dpo4, a result that was confirmed by tandem mass spectrometric analysis of full-length extension products. A crystal structure of the Dpo4 Glu(332) mutant and 8-oxoG:C pair revealed water-mediated hydrogen bonds between Glu(332) and the O-8 atom of 8-oxoG. The space normally occupied by Arg(332) side chain is empty in the crystal structures of the Ala(332) mutant. Two other crystal structures show that a Hoogsteen base pair is formed between 8-oxoG and A in the active site of both Glu(332) and Ala(332) mutants. These results support the view that a bond between Arg(332) and 8-oxoG plays a role in determining the fidelity and efficiency of Dpo4-catalyzed bypass of the lesion.

==About this Structure==
2UVU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVU OCA]. 

==Reference==
Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4., Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP, J Biol Chem. 2007 Jul 6;282(27):19831-43. Epub 2007 Apr 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17468100 17468100]
[[Category: DNA-directed DNA polymerase]]
[[Category: Single protein]]
[[Category: Sulfolobus solfataricus]]
[[Category: Egli, M.]]
[[Category: Irimia, A.]]
[[Category: CA]]
[[Category: DGT]]
[[Category: 7]]
[[Category: 8-dihydro-8-oxodeoxyguanosine]]
[[Category: dna damage]]
[[Category: dna repair]]
[[Category: dna replication]]
[[Category: dna- binding]]
[[Category: dna-binding]]
[[Category: dna-directed dna polymerase]]
[[Category: magnesium]]
[[Category: metal- binding]]
[[Category: metal-binding]]
[[Category: mutator protein]]
[[Category: nucleotidyltransferase]]
[[Category: p2 dna polymerase iv]]
[[Category: transferase]]
[[Category: translesion dna polymerase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:51:18 2008''