View source for 2lnl

==Structure of human CXCR1 in phospholipid bilayers==
<StructureSection load='2lnl' size='340' side='right' caption='[[2lnl]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lnl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNL FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CXCR1, CMKAR1, IL8RA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnl OCA], [http://pdbe.org/2lnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lnl RCSB], [http://www.ebi.ac.uk/pdbsum/2lnl PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CXCR1_HUMAN CXCR1_HUMAN]] Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. 
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== Publication Abstract from PubMed ==
CXCR1 is one of two high-affinity receptors for the CXC chemokine interleukin-8 (IL-8), a major mediator of immune and inflammatory responses implicated in many disorders, including tumour growth. IL-8, released in response to inflammatory stimuli, binds to the extracellular side of CXCR1. The ligand-activated intracellular signalling pathways result in neutrophil migration to the site of inflammation. CXCR1 is a class A, rhodopsin-like G-protein-coupled receptor (GPCR), the largest class of integral membrane proteins responsible for cellular signal transduction and targeted as drug receptors. Despite its importance, the molecular mechanism of CXCR1 signal transduction is poorly understood owing to the limited structural information available. Recent structural determination of GPCRs has advanced by modifying the receptors with stabilizing mutations, insertion of the protein T4 lysozyme and truncations of their amino acid sequences, as well as addition of stabilizing antibodies and small molecules that facilitate crystallization in cubic phase monoolein mixtures. The intracellular loops of GPCRs are crucial for G-protein interactions, and activation of CXCR1 involves both amino-terminal residues and extracellular loops. Our previous nuclear magnetic resonance studies indicate that IL-8 binding to the N-terminal residues is mediated by the membrane, underscoring the importance of the phospholipid bilayer for physiological activity. Here we report the three-dimensional structure of human CXCR1 determined by NMR spectroscopy. The receptor is in liquid crystalline phospholipid bilayers, without modification of its amino acid sequence and under physiological conditions. Features important for intracellular G-protein activation and signal transduction are revealed. The structure of human CXCR1 in a lipid bilayer should help to facilitate the discovery of new compounds that interact with GPCRs and combat diseases such as breast cancer.

Structure of the chemokine receptor CXCR1 in phospholipid bilayers.,Park SH, Das BB, Casagrande F, Tian Y, Nothnagel HJ, Chu M, Kiefer H, Maier K, De Angelis AA, Marassi FM, Opella SJ Nature. 2012 Nov 29;491(7426):779-83. doi: 10.1038/nature11580. Epub 2012 Oct 21. PMID:23086146<ref>PMID:23086146</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[CXC chemokine receptor|CXC chemokine receptor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Angelis, A De]]
[[Category: Casagrande, F]]
[[Category: Chu, M]]
[[Category: Das, B B]]
[[Category: Kiefer, H]]
[[Category: Maier, K]]
[[Category: Marassi, F M]]
[[Category: Nothnagel, H]]
[[Category: Opella, S J]]
[[Category: Park, S]]
[[Category: Chemokine]]
[[Category: G protein coupled receptor]]
[[Category: Gpcr]]
[[Category: Membrane protein]]
[[Category: Phospholipid]]
[[Category: Signaling]]
[[Category: Signaling protein]]
[[Category: Transmembrane]]