View source for 1gjr

[[Image:1gjr.gif|left|200px]]<br />
<applet load="1gjr" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1gjr, resolution 2.1&Aring;" />
'''FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION'''<br />

==Overview==
The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyses the production, of NADPH in photosynthesis. The three-dimensional structure of FNR, presents two distinct domains, one for binding of the FAD prosthetic group, and the other for NADP+ binding. In spite of extensive experiments and, different crystallographic approaches, many aspects about how the NADP+, substrate binds to FNR and how the hydride ion is transferred from FAD to, NADP+ remain unclear. The structure of an FNR:NADP+ complex from Anabaena, has been determined by X-ray diffraction analysis of the cocrystallised, units to 2.1 A resolution. Structural perturbation of FNR induced by, complex formation produces a narrower cavity in which the 2'-phospho-AMP, and pyrophosphate portions of the NADP+ are perfectly bound. In addition, the nicotinamide mononucleotide moiety is placed in a new pocket created, near the FAD cofactor with the ribose being in a tight conformation. The, crystal structure of this FNR:NADP+ complex obtained by cocrystallisation, displays NADP+ in an unusual conformation and can be considered as an, intermediate state in the process of coenzyme recognition and binding., Structural analysis and comparison with previously reported complexes, allow us to postulate a mechanism which would permit efficient hydride, transfer to occur. Besides, this structure gives new insights into the, postulated formation of the ferredoxin:FNR:NADP+ ternary complex by, prediction of new intermolecular interactions, which could only exist, after FNR:NADP+ complex formation. Finally, structural comparison with the, members of the broad FNR structural family also provides an explanation, for the high specificity exhibited by FNR for NADP+/H versus NAD+/H.

==About this Structure==
1GJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.] with FAD and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Structure known Active Site: FAD. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GJR OCA]. 

==Reference==
Mechanism of coenzyme recognition and binding revealed by crystal structure analysis of ferredoxin-NADP+ reductase complexed with NADP+., Hermoso JA, Mayoral T, Faro M, Gomez-Moreno C, Sanz-Aparicio J, Medina M, J Mol Biol. 2002 Jun 21;319(5):1133-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12079352 12079352]
[[Category: Anabaena sp.]]
[[Category: Ferredoxin--NADP(+) reductase]]
[[Category: Single protein]]
[[Category: Gomez-Moreno, C.]]
[[Category: Hermoso, J.A.]]
[[Category: Mayoral, T.]]
[[Category: Medina, M.]]
[[Category: Sanz-Aparicio, J.]]
[[Category: FAD]]
[[Category: NAP]]
[[Category: fad]]
[[Category: flavoprotein]]
[[Category: fnr]]
[[Category: nadp]]
[[Category: nadp reductase]]
[[Category: oxidoreductase]]

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