View source for 2mkc

==Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex==
<StructureSection load='2mkc' size='340' side='right' caption='[[2mkc]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mkc]] is a 3 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MKC FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mkc OCA], [http://pdbe.org/2mkc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mkc RCSB], [http://www.ebi.ac.uk/pdbsum/2mkc PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/IST3_YEAST IST3_YEAST]] Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.<ref>PMID:11287609</ref> <ref>PMID:15565172</ref> <ref>PMID:14973223</ref>  [[http://www.uniprot.org/uniprot/CWC26_YEAST CWC26_YEAST]] Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.<ref>PMID:8657162</ref> <ref>PMID:11452010</ref> <ref>PMID:12871902</ref> <ref>PMID:15565172</ref>  [[http://www.uniprot.org/uniprot/PML1_YEAST PML1_YEAST]] Required for efficient splicing and pre-mRNA nuclear retention.<ref>PMID:15565172</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The precursor mRNA (pre-mRNA) retention and splicing (RES) complex is a spliceosomal complex that is present in yeast and humans and is important for RNA splicing and retention of unspliced pre-mRNA. Here, we present the solution NMR structure of the RES core complex from Saccharomyces cerevisiae. Complex formation leads to an intricate folding of three components-Snu17p, Bud13p and Pml1p-that stabilizes the RNA-recognition motif (RRM) fold of Snu17p and increases binding affinity in tertiary interactions between the components by more than 100-fold compared to that in binary interactions. RES interacts with pre-mRNA within the spliceosome, and through the assembly of the RES core complex RNA binding efficiency is increased. The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome.

Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.,Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446<ref>PMID:25218446</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mkc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Becker, S]]
[[Category: Luhrmann, R]]
[[Category: Munari, F]]
[[Category: Schneider, C]]
[[Category: Trowitzsch, S]]
[[Category: Wahl, M C]]
[[Category: Wysoczanski, P]]
[[Category: Xiang, S]]
[[Category: Zweckstetter, M]]
[[Category: Bud13p]]
[[Category: Cooperativity]]
[[Category: Heterotrimer]]
[[Category: Ist3p]]
[[Category: Pml1p]]
[[Category: Protein binding]]
[[Category: Re]]
[[Category: Rrm]]
[[Category: Snu17p]]
[[Category: Spliceosome]]
[[Category: Splicing]]