View source for 1wby

==CRYSTAL STRUCTURES OF MURINE MHC CLASS I H-2 DB AND KB MOLECULES IN COMPLEX WITH CTL EPITOPES FROM INFLUENZA A VIRUS: IMPLICATIONS FOR TCR REPERTOIRE SELECTION AND IMMUNODOMINANCE==
<StructureSection load='1wby' size='340' side='right' caption='[[1wby]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wby]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WBY FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bii|1bii]], [[1bqh|1bqh]], [[1bz9|1bz9]], [[1cd1|1cd1]], [[1ddh|1ddh]], [[1ffn|1ffn]], [[1ffo|1ffo]], [[1ffp|1ffp]], [[1fg2|1fg2]], [[1fo0|1fo0]], [[1fzj|1fzj]], [[1fzk|1fzk]], [[1fzm|1fzm]], [[1fzo|1fzo]], [[1g6r|1g6r]], [[1g7p|1g7p]], [[1g7q|1g7q]], [[1hoc|1hoc]], [[1inq|1inq]], [[1jpf|1jpf]], [[1jpg|1jpg]], [[1juf|1juf]], [[1k8d|1k8d]], [[1kbg|1kbg]], [[1kj2|1kj2]], [[1kj3|1kj3]], [[1kpu|1kpu]], [[1kpv|1kpv]], [[1l6q|1l6q]], [[1ld9|1ld9]], [[1ldp|1ldp]], [[1leg|1leg]], [[1lek|1lek]], [[1lk2|1lk2]], [[1mhc|1mhc]], [[1mwa|1mwa]], [[1n3n|1n3n]], [[1n59|1n59]], [[1n5a|1n5a]], [[1nam|1nam]], [[1nan|1nan]], [[1nez|1nez]], [[1osz|1osz]], [[1p1z|1p1z]], [[1p4l|1p4l]], [[1pqz|1pqz]], [[1qo3|1qo3]], [[1s7q|1s7q]], [[1s7r|1s7r]], [[1s7s|1s7s]], [[1s7t|1s7t]], [[1s7u|1s7u]], [[1s7v|1s7v]], [[1s7w|1s7w]], [[1s7x|1s7x]], [[1vac|1vac]], [[1vad|1vad]], [[2ckb|2ckb]], [[2mha|2mha]], [[2vaa|2vaa]], [[2vab|2vab]], [[1ce6|1ce6]], [[1wbx|1wbx]], [[1wbz|1wbz]], [[1qlf|1qlf]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wby OCA], [http://pdbe.org/1wby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wby RCSB], [http://www.ebi.ac.uk/pdbsum/1wby PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/HA11_MOUSE HA11_MOUSE]] Involved in the presentation of foreign antigens to the immune system. [[http://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/1wby_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytotoxic T lymphocyte (CTL) responses against influenza A virus in C57BL/6 mice are dominated by a small number of viral peptides among many that are capable of binding to major histocompatibility complex (MHC) class I molecules. The basis of this limited immune recognition is unknown. Here, we present X-ray structures of MHC class I molecules in complex with two immunodominant epitopes (PA(224-233)/D(b) and PB1(703-711)/K(b)) and one non-immunogenic epitope (HA(468-477)/D(b)) of the influenza A virus. The immunodominant peptides are each characterized by a bulge at the C terminus, lifting P6 and P7 residues out of the MHC groove, presenting featured structural elements to T-cell receptors (TCRs). Immune recognition of PA(224-233)/D(b) will focus largely on the exposed P7 arginine residue. In contrast, the non-immunogenic HA(468-477) peptide lacks prominent features in this C-terminal bulge. In the K(b)-bound PB1(703-711) epitope, the bulge results from a non-canonical binding motif, such that the mode of presentation of this peptide strongly resembles that of D(b)-bound peptides. Given that PA(224-233)/D(b), PB1(703-711)/K(b) and the previously defined NP(366-374)/D(b) epitopes dominate the primary response to influenza A virus in C57BL/6 mice, our findings indicate that residues of the C-terminal bulge are important in selection of the immunodominant CTL repertoire.

Crystal structures of murine MHC Class I H-2 D(b) and K(b) molecules in complex with CTL epitopes from influenza A virus: implications for TCR repertoire selection and immunodominance.,Meijers R, Lai CC, Yang Y, Liu JH, Zhong W, Wang JH, Reinherz EL J Mol Biol. 2005 Feb 4;345(5):1099-110. Epub 2004 Dec 8. PMID:15644207<ref>PMID:15644207</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1wby" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-2 microglobulin|Beta-2 microglobulin]]
*[[Major histocompatibility complex|Major histocompatibility complex]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lk3 transgenic mice]]
[[Category: Lai, C]]
[[Category: Liu, J]]
[[Category: Meijers, R]]
[[Category: Reinherz, E L]]
[[Category: Wang, J]]
[[Category: Yang, Y]]
[[Category: Zhong, W]]
[[Category: Immmune system]]
[[Category: Immune system]]
[[Category: Influenza peptide]]
[[Category: Mhc class i]]
[[Category: Pa224]]