View source for 1nbm

[[Image:1nbm.jpg|left|200px]]

 {{Structure
|PDB= 1nbm |SIZE=350|CAPTION= <scene name='initialview01'>1nbm</scene>, resolution 3.0&Aring;
|SITE= <scene name='pdbsite=CA1:The+Carboxylate+Group+Of+GLU+Residue+Is+Believed+To+Acti+...'>CA1</scene>, <scene name='pdbsite=CA2:The+Carboxylate+Group+Of+GLU+Residue+Is+Believed+To+Acti+...'>CA2</scene>, <scene name='pdbsite=CA3:The+Carboxylate+Group+Of+GLU+Residue+Is+Believed+To+Acti+...'>CA3</scene>, <scene name='pdbsite=PL1:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL1</scene>, <scene name='pdbsite=PL2:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL2</scene>, <scene name='pdbsite=PL3:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL3</scene>, <scene name='pdbsite=PL4:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL4</scene>, <scene name='pdbsite=PL5:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL5</scene>, <scene name='pdbsite=PL6:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL6</scene> and <scene name='pdbsite=PL7:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PL7</scene>
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene> and <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] 
|GENE= 
}}

'''THE STRUCTURE OF BOVINE F1-ATPASE COVALENTLY INHIBITED WITH 4-CHLORO-7-NITROBENZOFURAZAN'''


==Overview==
BACKGROUND: F1-ATPase is the globular domain of F1F0-ATP synthase that catalyses the hydrolysis of ATP to ADP and phosphate. The crystal structure of bovine F1-ATPase has been determined previously to 2.8 A resolution. The enzyme comprises five different subunits in the stoichiometry alpha 3 beta 3 gamma delta epsilon; the three catalytic beta subunits alternate with the three alpha subunits around the centrally located single gamma subunit. To understand more about the catalytic mechanisms, F1-ATPase was inhibited by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) and the structure of the inhibited complex (F1-NBD) determined by X-ray crystallography. RESULTS: In the structure the three beta subunits adopt a different conformation with different nucleotide occupancy. NBD-Cl reacts with the phenolic oxygen of Tyr311 of the beta E subunit, which contains no bound nucleotide. The two other catalytic subunits beta TP and beta DP contain bound adenylyl-imidodiphosphate (AMP-PNP) and ADP, respectively. The binding site of the NBD moiety does not overlap with the regions of beta E that form the nucleotide-binding pocket in subunits beta TP and beta DP nor does it occlude the nucleotide-binding site. Catalysis appears to be inhibited because neither beta TP nor beta DP can accommodate a Tyr311 residue bearing an NBD group. CONCLUSIONS: The results presented here are consistent with a rotary catalytic mechanism of ATP synthesis and hydrolysis, which requires the sequential and concerted participation of all three catalytic sites. NBD-Cl inhibits the enzyme by preventing the modified subunit from adopting a conformation that is essential for catalysis to proceed.

==About this Structure==
1NBM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBM OCA]. 

==Reference==
Bovine F1-ATPase covalently inhibited with 4-chloro-7-nitrobenzofurazan: the structure provides further support for a rotary catalytic mechanism., Orriss GL, Leslie AG, Braig K, Walker JE, Structure. 1998 Jul 15;6(7):831-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9687365 9687365]
[[Category: Bos taurus]]
[[Category: Protein complex]]
[[Category: Transferred entry: 3 6.3 14]]
[[Category: Braig, K.]]
[[Category: Leslie, A G.W.]]
[[Category: Orriss, G L.]]
[[Category: Walker, J E.]]
[[Category: ADP]]
[[Category: ATP]]
[[Category: MG]]
[[Category: PO4]]
[[Category: 4-chloro-7-nitrobenzofurazan]]
[[Category: atp synthase]]
[[Category: f1-atpase]]
[[Category: f1fo atp synthase]]
[[Category: inhibition]]

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