View source for 1in4

[[Image:1in4.gif|left|200px]]

 {{Structure
|PDB= 1in4 |SIZE=350|CAPTION= <scene name='initialview01'>1in4</scene>, resolution 1.60&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>
|ACTIVITY= 
|GENE= RuvB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
}}

'''THERMOTOGA MARITIMA RUVB HOLLIDAY JUNCTION BRANCH MIGRATION MOTOR'''


==Overview==
The RuvB hexamer is the chemomechanical motor of the RuvAB complex that migrates Holliday junction branch-points in DNA recombination and the rescue of stalled DNA replication forks. The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant structures reveal that RuvB is an ATPase-associated with diverse cellular activities (AAA+-class ATPase) with a winged-helix DNA-binding domain. The RuvB-ADP complex structure and mutagenesis suggest how AAA+-class ATPases couple nucleotide binding and hydrolysis to interdomain conformational changes and asymmetry within the RuvB hexamer implied by the crystallographic packing and small-angle X-ray scattering in solution. ATP-driven domain motion is positioned to move double-stranded DNA through the hexamer and drive conformational changes between subunits by altering the complementary hydrophilic protein- protein interfaces. Structural and biochemical analysis of five motifs in the protein suggest that ATP binding is a strained conformation recognized both by sensors and the Walker motifs and that intersubunit activation occurs by an arginine finger motif reminiscent of the GTPase-activating proteins. Taken together, these results provide insights into how RuvB functions as a motor for branch migration of Holliday junctions.

==About this Structure==
1IN4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN4 OCA]. 

==Reference==
Structure and mechanism of the RuvB Holliday junction branch migration motor., Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA, J Mol Biol. 2001 Aug 10;311(2):297-310. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11478862 11478862]
[[Category: Single protein]]
[[Category: Thermotoga maritima]]
[[Category: Putnam, C D.]]
[[Category: Tainer, J A.]]
[[Category: ACT]]
[[Category: ADP]]
[[Category: CO]]
[[Category: HEZ]]
[[Category: aaa+-class atpase]]
[[Category: arginine finger]]
[[Category: atp hydrolysis]]
[[Category: branch migration]]
[[Category: dna translocase]]
[[Category: holliday junction]]
[[Category: sensor 1]]
[[Category: sensor 2]]
[[Category: walker some]]
[[Category: walker b]]
[[Category: winged-helix domain]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:14:13 2008''