View source for 1fyf

[[Image:1fyf.gif|left|200px]]

 {{Structure
|PDB= 1fyf |SIZE=350|CAPTION= <scene name='initialview01'>1fyf</scene>, resolution 1.65&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SSA:5&#39;-O-(N-(L-SERYL)-SULFAMOYL)ADENOSINE'>SSA</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Threonine--tRNA_ligase Threonine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.3 6.1.1.3] 
|GENE= 
}}

'''CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE COMPLEXED WITH A SERYL ADENYLATE ANALOG'''


==Overview==
Threonyl-tRNA synthetase, a class II synthetase, uses a unique zinc ion to discriminate against the isosteric valine at the activation step. The crystal structure of the enzyme with an analog of seryl adenylate shows that the noncognate serine cannot be fully discriminated at that step. We show that hydrolysis of the incorrectly formed ser-tRNA(Thr) is performed at a specific site in the N-terminal domain of the enzyme. The present study suggests that both classes of synthetases use effectively the ability of the CCA end of tRNA to switch between a hairpin and a helical conformation for aminoacylation and editing. As a consequence, the editing mechanism of both classes of synthetases can be described as mirror images, as already seen for tRNA binding and amino acid activation.

==About this Structure==
1FYF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYF OCA]. 

==Reference==
Transfer RNA-mediated editing in threonyl-tRNA synthetase. The class II solution to the double discrimination problem., Dock-Bregeon A, Sankaranarayanan R, Romby P, Caillet J, Springer M, Rees B, Francklyn CS, Ehresmann C, Moras D, Cell. 2000 Dec 8;103(6):877-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11136973 11136973]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Threonine--tRNA ligase]]
[[Category: Dock-Bregeon, A C.]]
[[Category: Moras, D.]]
[[Category: Sankaranarayanan, R.]]
[[Category: SSA]]
[[Category: ZN]]
[[Category: adenylate analog]]
[[Category: amino acid recognition]]
[[Category: deletion mutant]]
[[Category: trna-synthetase]]
[[Category: zinc ion]]

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