View source for 1fdi

[[Image:1fdi.gif|left|200px]]<br />
<applet load="1fdi" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1fdi, resolution 2.9&Aring;" />
'''OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI COMPLEXED WITH THE INHIBITOR NITRITE'''<br />

==Overview==
Formate dehydrogenase H from Escherichia coli contains selenocysteine, (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD), cofactors, and an Fe4S4 cluster at the active site and catalyzes the, two-electron oxidation of formate to carbon dioxide. The crystal, structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate, dehydrogenase H (with and without bound inhibitor) and the reduced, [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain, alphabeta structure with the molybdenum directly coordinated to selenium, and both MGD cofactors. These structures suggest a reaction mechanism that, directly involves SeCys140 and His141 in proton abstraction and the, molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron, transfer.

==About this Structure==
1FDI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NO2, SF4, MGD and 6MO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Structure known Active Sites: FS4 and MO4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FDI OCA]. 

==Reference==
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9036855 9036855]
[[Category: Escherichia coli]]
[[Category: Formate dehydrogenase]]
[[Category: Single protein]]
[[Category: Boyington, J.C.]]
[[Category: Sun, P.D.]]
[[Category: 6MO]]
[[Category: MGD]]
[[Category: NO2]]
[[Category: SF4]]
[[Category: anaerobic]]
[[Category: dehydrogenase]]
[[Category: fe4s4]]
[[Category: formate]]
[[Category: iron sulfur cluster]]
[[Category: mgd]]
[[Category: molybdenum]]
[[Category: molybdopterin]]
[[Category: molybdopterin guanine dinucleotide]]
[[Category: mpt]]
[[Category: oxidoreductase]]
[[Category: secys]]
[[Category: selenium]]
[[Category: selenocysteine]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:29:00 2007''