View source for 2znc

[[Image:2znc.gif|left|200px]]

 {{Structure
|PDB= 2znc |SIZE=350|CAPTION= <scene name='initialview01'>2znc</scene>, resolution 2.8&Aring;
|SITE= <scene name='pdbsite=ZN:Zn+Binding+Site'>ZN</scene>
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] 
|GENE= MURINE CAIV ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
}}

'''MURINE CARBONIC ANHYDRASE IV'''


==Overview==
Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. We have determined the 2.8-angstroms resolution crystal structure of a truncated, soluble form of recombinant murine CAIV. We have also determined the structure of its complex with a drug used for glaucoma therapy, the sulfonamide inhibitor brinzolamide (Azopt). The overall structure of murine CAIV is generally similar to that of human CAIV; however, some local structural differences are found in the active site resulting from amino acid sequence differences in the "130's segment" and the residue-63 loop (these may affect the nearby catalytic proton shuttle, His-64). Similar to human CAIV, the C-terminus of murine CAIV is surrounded by a substantial electropositive surface potential that may stabilize the interaction with the phospholipid membrane. Binding interactions observed for brinzolamide rationalize the generally weaker affinity of inhibitors used in glaucoma therapy toward CAIV compared with CAII.

==About this Structure==
2ZNC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZNC OCA]. 

==Reference==
Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination., Stams T, Chen Y, Boriack-Sjodin PA, Hurt JD, Liao J, May JA, Dean T, Laipis P, Silverman DN, Christianson DW, Protein Sci. 1998 Mar;7(3):556-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9541386 9541386]
[[Category: Carbonate dehydratase]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Chen, Y.]]
[[Category: Christianson, D W.]]
[[Category: Stams, T.]]
[[Category: ZN]]
[[Category: lyase]]
[[Category: membrane]]
[[Category: murine]]
[[Category: zinc]]

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