View source for 2pzs

[[Image:2pzs.gif|left|200px]]

 {{Structure
|PDB= 2pzs |SIZE=350|CAPTION= <scene name='initialview01'>2pzs</scene>, resolution 2.60&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] 
|GENE= 2, gp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=221993 Vibrio phage f237])
}}

'''Phi29 DNA polymerase complexed with primer-template DNA (post-translocation binary complex)'''


==Overview==
Replicative DNA polymerases (DNAPs) move along template DNA in a processive manner. The structural basis of the mechanism of translocation has been better studied in the A-family of polymerases than in the B-family of replicative polymerases. To address this issue, we have determined the X-ray crystal structures of phi29 DNAP, a member of the protein-primed subgroup of the B-family of polymerases, complexed with primer-template DNA in the presence or absence of the incoming nucleoside triphosphate, the pre- and post-translocated states, respectively. Comparison of these structures reveals a mechanism of translocation that appears to be facilitated by the coordinated movement of two conserved tyrosine residues into the insertion site. This differs from the mechanism employed by the A-family polymerases, in which a conserved tyrosine moves into the templating and insertion sites during the translocation step. Polymerases from the two families also interact with downstream single-stranded template DNA in very different ways.

==About this Structure==
2PZS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZS OCA]. 

==Reference==
Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases., Berman AJ, Kamtekar S, Goodman JL, Lazaro JM, de Vega M, Blanco L, Salas M, Steitz TA, EMBO J. 2007 Jul 25;26(14):3494-505. Epub 2007 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17611604 17611604]
[[Category: DNA-directed DNA polymerase]]
[[Category: Single protein]]
[[Category: Vibrio phage f237]]
[[Category: Berman, A J.]]
[[Category: Blanco, L.]]
[[Category: Goodman, J L.]]
[[Category: Kamtekar, S.]]
[[Category: Lazaro, J M.]]
[[Category: Salas, M.]]
[[Category: Steitz, T A.]]
[[Category: Vega, M de.]]
[[Category: protein-dna complex]]

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