View source for 2nwl

[[Image:2nwl.gif|left|200px]]

 {{Structure
|PDB= 2nwl |SIZE=350|CAPTION= <scene name='initialview01'>2nwl</scene>, resolution 2.960&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene> and <scene name='pdbligand=PLM:PALMITIC ACID'>PLM</scene>
|ACTIVITY= 
|GENE= 
}}

'''Crystal structure of GltPh in complex with L-Asp'''


==Overview==
Secondary transporters are integral membrane proteins that catalyse the movement of substrate molecules across the lipid bilayer by coupling substrate transport to one or more ion gradients, thereby providing a mechanism for the concentrative uptake of substrates. Here we describe crystallographic and thermodynamic studies of Glt(Ph), a sodium (Na+)-coupled aspartate transporter, defining sites for aspartate, two sodium ions and d,l-threo-beta-benzyloxyaspartate, an inhibitor. We further show that helical hairpin 2 is the extracellular gate that controls access of substrate and ions to the internal binding sites. At least two sodium ions bind in close proximity to the substrate and these sodium-binding sites, together with the sodium-binding sites in another sodium-coupled transporter, LeuT, define an unwound alpha-helix as the central element of the ion-binding motif, a motif well suited to the binding of sodium and to participation in conformational changes that accompany ion binding and unbinding during the transport cycle.

==About this Structure==
2NWL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NWL OCA]. 

==Reference==
Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter., Boudker O, Ryan RM, Yernool D, Shimamoto K, Gouaux E, Nature. 2007 Jan 25;445(7126):387-93. Epub 2007 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17230192 17230192]
[[Category: Pyrococcus horikoshii]]
[[Category: Single protein]]
[[Category: Boudker, O.]]
[[Category: Gouaux, E.]]
[[Category: Ryan, R.]]
[[Category: Shimamoto, K.]]
[[Category: Yernool, D.]]
[[Category: ASP]]
[[Category: PLM]]
[[Category: alpha helical]]
[[Category: helical hairpin]]
[[Category: membrane protein]]
[[Category: unwound region]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:52:05 2008''