View source for 2nps

[[Image:2nps.jpg|left|200px]]

 {{Structure
|PDB= 2nps |SIZE=350|CAPTION= <scene name='initialview01'>2nps</scene>, resolution 2.5&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= Vamp4_predicted ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), syntaxin 13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), Vti1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), STX6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
}}

'''Crystal Structure of the Early Endosomal SNARE Complex'''


==Overview==
SNARE proteins mediate membrane fusion in eukaryotic cells. They contain conserved SNARE motifs that are usually located adjacent to a C-terminal transmembrane domain. SNARE motifs spontaneously assemble into four helix bundles, with each helix belonging to a different subfamily. Liposomes containing SNAREs spontaneously fuse with each other, but it is debated how the SNAREs are distributed between the membranes. Here, we report that the SNAREs mediating homotypic fusion of early endosomes fuse liposomes in five out of seven possible combinations, in contrast to previously studied SNAREs involved in heterotypic fusion events. The crystal structure of the early endosomal SNARE complex resembles that of the neuronal and late endosomal complexes, but differs in surface side-chain interactions. We conclude that homotypic fusion reactions may proceed with multiple SNARE topologies, suggesting that the conserved SNARE structure allows for flexibility in the initial interactions needed for fusion.

==About this Structure==
2NPS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPS OCA]. 

==Reference==
Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies., Zwilling D, Cypionka A, Pohl WH, Fasshauer D, Walla PJ, Wahl MC, Jahn R, EMBO J. 2007 Jan 10;26(1):9-18. Epub 2006 Dec 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17159904 17159904]
[[Category: Homo sapiens]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Rattus norvegicus]]
[[Category: Cypionka, A.]]
[[Category: Fasshauer, D.]]
[[Category: Jahn, R.]]
[[Category: Pohl, W H.]]
[[Category: Wahl, M C.]]
[[Category: Walla, P J.]]
[[Category: Zwilling, D.]]
[[Category: early endosomal snare complex]]
[[Category: snare complex]]
[[Category: syntaxin 13]]
[[Category: syntaxin 6]]
[[Category: vamp4]]
[[Category: vesicle fusion]]
[[Category: vti1a]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:49:34 2008''