View source for 2jnf

[[Image:2jnf.jpg|left|200px]]

 {{Structure
|PDB= 2jnf |SIZE=350|CAPTION= <scene name='initialview01'>2jnf</scene>
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212017 Lethocerus indicus])
}}

'''Solution structure of fly troponin C, isoform F1'''


==Overview==
To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.

==About this Structure==
2JNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. 

==Reference==
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637342 17637342]
[[Category: Lethocerus indicus]]
[[Category: Single protein]]
[[Category: Bullard, B.]]
[[Category: Nicola, G F.De.]]
[[Category: Pastore, A.]]
[[Category: ef-hand]]
[[Category: lethocerus indicus]]
[[Category: metal binding protein]]
[[Category: stretch activated muscle contraction]]
[[Category: troponin c]]

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