View source for 2imc

[[Image:2imc.jpg|left|200px]]

 {{Structure
|PDB= 2imc |SIZE=350|CAPTION= <scene name='initialview01'>2imc</scene>, resolution 2.00&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] 
|GENE= botA, atx, bna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])
}}

'''Clostridium botulinum Neurotoxin Serotype A Light Chain, Residues 1-424'''


==Overview==
The potential for the use of Clostridial neurotoxins as bioweapons makes the development of small-molecule inhibitors of these deadly toxins a top priority. Recently, screening of a random hydroxamate library identified a small-molecule inhibitor of C. botulinum Neurotoxin Serotype A Light Chain (BoNT/A-LC), 4-chlorocinnamic hydroxamate, a derivative of which has been shown to have in vivo efficacy in mice and no toxicity. We describe the X-ray crystal structures of BoNT/A-LC in complexes with two potent small-molecule inhibitors. The structures of the enzyme with 4-chlorocinnamic hydroxamate or 2,4-dichlorocinnamic hydroxamate bound are compared to the structure of the enzyme complexed with L-arginine hydroxamate, an inhibitor with modest affinity. Taken together, this suite of structures provides surprising insights into the BoNT/A-LC active site, including unexpected conformational flexibility at the S1' site that changes the electrostatic environment of the binding pocket. Information gained from these structures will inform the design and optimization of more effective small-molecule inhibitors of BoNT/A-LC.

==About this Structure==
2IMC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMC OCA]. 

==Reference==
Structures of Clostridium botulinum Neurotoxin Serotype A Light Chain complexed with small-molecule inhibitors highlight active-site flexibility., Silvaggi NR, Boldt GE, Hixon MS, Kennedy JP, Tzipori S, Janda KD, Allen KN, Chem Biol. 2007 May;14(5):533-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17524984 17524984]
[[Category: Bontoxilysin]]
[[Category: Clostridium botulinum]]
[[Category: Single protein]]
[[Category: Allen, K N.]]
[[Category: Silvaggi, N R.]]
[[Category: ZN]]
[[Category: clostridium botulinum neurotoxin serotype some]]
[[Category: protease inhibitor]]
[[Category: substrate specificity]]

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