View source for 4wab

==Crystal structure of mPGES1 solved by native-SAD phasing==
<StructureSection load='4wab' size='340' side='right' caption='[[4wab]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wab]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WAB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WAB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=LVJ:2-[[2,6-BIS(CHLORANYL)-3-[(2,2-DIMETHYLPROPANOYLAMINO)METHYL]PHENYL]AMINO]-1-METHYL-6-(2-METHYL-2-OXIDANYL-PROPOXY)-N-[2,2,2-TRIS(FLUORANYL)ETHYL]BENZIMIDAZOLE-5-CARBOXAMIDE'>LVJ</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-E_synthase Prostaglandin-E synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.3 5.3.99.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wab RCSB], [http://www.ebi.ac.uk/pdbsum/4wab PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PTGES_HUMAN PTGES_HUMAN]] Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).<ref>PMID:18682561</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

Fast native-SAD phasing for routine macromolecular structure determination.,Weinert T, Olieric V, Waltersperger S, Panepucci E, Chen L, Zhang H, Zhou D, Rose J, Ebihara A, Kuramitsu S, Li D, Howe N, Schnapp G, Pautsch A, Bargsten K, Prota AE, Surana P, Kottur J, Nair DT, Basilico F, Cecatiello V, Pasqualato S, Boland A, Weichenrieder O, Wang B, Steinmetz MO, Caffrey M, Wang M Nat Methods. 2014 Dec 15. doi: 10.1038/nmeth.3211. PMID:25506719<ref>PMID:25506719</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Prostaglandin-E synthase]]
[[Category: Caffrey, M]]
[[Category: Howe, N]]
[[Category: Li, D]]
[[Category: Wang, M]]
[[Category: Weinert, T]]
[[Category: Anomalous dispersion]]
[[Category: Cancer]]
[[Category: Drug target]]
[[Category: In meso crystallization]]
[[Category: Inflammation]]
[[Category: Inhibitor]]
[[Category: Isomerase]]
[[Category: Leukotriene c4 synthase]]
[[Category: Lipid metabolism]]
[[Category: Mapag]]
[[Category: Membrane protein]]
[[Category: Membrane-associated proteins in eicosanoid and glutathione metabolism]]
[[Category: Microcrystal]]
[[Category: Mpges1]]
[[Category: Native-sad]]
[[Category: Pain]]
[[Category: S-sad]]
[[Category: Sulfur-sad]]