View source for 2rlf

==Proton Channel M2 from Influenza A in complex with inhibitor rimantadine==
<StructureSection load='2rlf' size='340' side='right' caption='[[2rlf]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2rlf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RLF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RIM:RIMANTADINE'>RIM</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10239 Viruses])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rlf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rlf RCSB], [http://www.ebi.ac.uk/pdbsum/2rlf PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/M2_IAUDO M2_IAUDO]] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>  
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== Publication Abstract from PubMed ==
The integral membrane protein M2 of influenza virus forms pH-gated proton channels in the viral lipid envelope. The low pH of an endosome activates the M2 channel before haemagglutinin-mediated fusion. Conductance of protons acidifies the viral interior and thereby facilitates dissociation of the matrix protein from the viral nucleoproteins--a required process for unpacking of the viral genome. In addition to its role in release of viral nucleoproteins, M2 in the trans-Golgi network (TGN) membrane prevents premature conformational rearrangement of newly synthesized haemagglutinin during transport to the cell surface by equilibrating the pH of the TGN with that of the host cell cytoplasm. Inhibiting the proton conductance of M2 using the anti-viral drug amantadine or rimantadine inhibits viral replication. Here we present the structure of the tetrameric M2 channel in complex with rimantadine, determined by NMR. In the closed state, four tightly packed transmembrane helices define a narrow channel, in which a 'tryptophan gate' is locked by intermolecular interactions with aspartic acid. A carboxy-terminal, amphipathic helix oriented nearly perpendicular to the transmembrane helix forms an inward-facing base. Lowering the pH destabilizes the transmembrane helical packing and unlocks the gate, admitting water to conduct protons, whereas the C-terminal base remains intact, preventing dissociation of the tetramer. Rimantadine binds at four equivalent sites near the gate on the lipid-facing side of the channel and stabilizes the closed conformation of the pore. Drug-resistance mutations are predicted to counter the effect of drug binding by either increasing the hydrophilicity of the pore or weakening helix-helix packing, thus facilitating channel opening.

Structure and mechanism of the M2 proton channel of influenza A virus.,Schnell JR, Chou JJ Nature. 2008 Jan 31;451(7178):591-5. PMID:18235503<ref>PMID:18235503</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Avian Influenza Neuraminidase%2C Tamiflu and Relenza|Avian Influenza Neuraminidase%2C Tamiflu and Relenza]]
*[[M2 protein|M2 protein]]
*[[Proton Channels|Proton Channels]]
*[[Rimantadine|Rimantadine]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Viruses]]
[[Category: Chou, J J]]
[[Category: Schnell, J R]]
[[Category: M2]]
[[Category: Proton channel]]
[[Category: Proton transport]]
[[Category: Rimantadine]]