View source for 2f1d

[[Image:2f1d.gif|left|200px]]

 {{Structure
|PDB= 2f1d |SIZE=350|CAPTION= <scene name='initialview01'>2f1d</scene>, resolution 3.00&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Imidazoleglycerol-phosphate_dehydratase Imidazoleglycerol-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.19 4.2.1.19] 
|GENE= 
}}

'''X-Ray Structure of imidazoleglycerol-phosphate dehydratase'''


==Overview==
The structure of A. thaliana imidazoleglycerol-phosphate dehydratase, an enzyme of histidine biosynthesis and a target for the triazole phosphonate herbicides, has been determined to 3.0 A resolution. The structure is composed of 24 identical subunits arranged in 432 symmetry and shows how the formation of a novel dimanganese cluster is crucial to the assembly of the active 24-mer from an inactive trimeric precursor and to the formation of the active site of the enzyme. Molecular modeling suggests that the substrate is bound to the manganese cluster as an imidazolate moiety that subsequently collapses to yield a diazafulvene intermediate. The mode of imidazolate recognition exploits pseudosymmetry at the active site arising from a combination of the assembly of the particle and the pseudosymmetry present in each subunit as a result of gene duplication. This provides an intriguing example of the role of evolution in the design of Nature's catalysts.

==About this Structure==
2F1D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1D OCA]. 

==Reference==
Structure and mechanism of imidazoleglycerol-phosphate dehydratase., Glynn SE, Baker PJ, Sedelnikova SE, Davies CL, Eadsforth TC, Levy CW, Rodgers HF, Blackburn GM, Hawkes TR, Viner R, Rice DW, Structure. 2005 Dec;13(12):1809-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16338409 16338409]
[[Category: Arabidopsis thaliana]]
[[Category: Imidazoleglycerol-phosphate dehydratase]]
[[Category: Single protein]]
[[Category: Baker, P J.]]
[[Category: Davies, C L.]]
[[Category: Eadsforth, T C.]]
[[Category: Glynn, S E.]]
[[Category: Rice, D W.]]
[[Category: Sedelnikova, S E.]]
[[Category: MN]]
[[Category: SO4]]
[[Category: herbicide]]
[[Category: histidine biosynthesis]]
[[Category: igpd]]
[[Category: manganese]]
[[Category: x-ray crystallography]]

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