View source for 3wjw

==Wild-type orotidine 5'-monophosphate decarboxylase from M. thermoautotrophicus complexed with 6-methyl-UMP==
<StructureSection load='3wjw' size='340' side='right' caption='[[3wjw]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wjw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Metth Metth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U6M:6-METHYLURIDINE+5-(DIHYDROGEN+PHOSPHATE)'>U6M</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wjx|3wjx]], [[3wjy|3wjy]], [[3wjz|3wjz]], [[3wk0|3wk0]], [[3wk1|3wk1]], [[3wk2|3wk2]], [[3wk3|3wk3]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pyrF, MTH_129 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 METTH])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Orotidine-5'-phosphate_decarboxylase Orotidine-5'-phosphate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.23 4.1.1.23] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wjw RCSB], [http://www.ebi.ac.uk/pdbsum/3wjw PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PYRF_METTH PYRF_METTH]] Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).[HAMAP-Rule:MF_01200_A] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Orotidine 5'-monophosphate decarboxylase (ODCase) accelerates the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) by 17 orders of magnitude. Eight new crystal structures with ligand analogues combined with computational analyses of the enzyme's short-lived intermediates and the intrinsic electronic energies to distort the substrate and other ligands improve our understanding of the still controversially discussed reaction mechanism. In their respective complexes, 6-methyl-UMP displays significant distortion of its methyl substituent bond, 6-amino-UMP shows the competition between the K72 and C6 substituents for a position close to D70, and the methyl and ethyl esters of OMP both induce rotation of the carboxylate group substituent out of the plane of the pyrimidine ring. Molecular dynamics and quantum mechanics/molecular mechanics computations of the enzyme-substrate complex also show the bond between the carboxylate group and the pyrimidine ring to be distorted, with the distortion contributing a 10-15% decrease of the DeltaDeltaG() value. These results are consistent with ODCase using both substrate distortion and transition-state stabilization, primarily exerted by K72, in its catalysis of the OMP decarboxylation reaction.

Substrate distortion contributes to the catalysis of orotidine 5'-monophosphate decarboxylase.,Fujihashi M, Ishida T, Kuroda S, Kotra LP, Pai EF, Miki K J Am Chem Soc. 2013 Nov 20;135(46):17432-43. doi: 10.1021/ja408197k. Epub 2013, Nov 11. PMID:24151964<ref>PMID:24151964</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Metth]]
[[Category: Orotidine-5'-phosphate decarboxylase]]
[[Category: Fujihashi, M]]
[[Category: Kuroda, S]]
[[Category: Miki, K]]
[[Category: Pai, E F]]
[[Category: Decarboxylase]]
[[Category: Lyase]]
[[Category: Protein-ligand complex]]
[[Category: Pyrimidine biosynthesis]]
[[Category: Tim barrel]]