View source for 2emt

[[Image:2emt.jpg|left|200px]]

 {{Structure
|PDB= 2emt |SIZE=350|CAPTION= <scene name='initialview01'>2emt</scene>, resolution 2.80&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
}}

'''Crystal Structure Analysis of the radixin FERM domain complexed with adhesion molecule PSGL-1'''


==Overview==
P-selectin glycoprotein ligand-1 (PSGL-1), an adhesion molecule with O-glycosylated extracellular sialomucins, is involved in leukocyte inflammatory responses. On activation, ezrin-radixin-moesin (ERM) proteins mediate the redistribution of PSGL-1 on polarized cell surfaces to facilitate binding to target molecules. ERM proteins recognize a short binding motif, Motif-1, conserved in cytoplasmic tails of adhesion molecules, whereas PSGL-1 lacks Motif-1 residues important for binding to ERM proteins. The crystal structure of the complex between the radixin FERM domain and a PSGL-1 juxtamembrane peptide reveals that the peptide binds the groove of FERM subdomain C by forming a beta-strand associated with strand beta5C, followed by a loop flipped out towards the solvent. The Motif-1 3(10) helix present in the FERM-ICAM-2 complex is absent in PSGL-1 given the absence of a critical Motif-1 alanine residue, and PSGL-1 reduces its contact area with subdomain C. Non-conserved positions are occupied by large residues Met9 and His8, which stabilize peptide conformation and enhance groove binding. Non-conserved residues play an important role in compensating for loss of binding energy resulting from the absence of conserved residues important for binding.

==About this Structure==
2EMT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EMT OCA]. 

==Reference==
Structural basis of PSGL-1 binding to ERM proteins., Takai Y, Kitano K, Terawaki S, Maesaki R, Hakoshima T, Genes Cells. 2007 Dec;12(12):1329-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18076570 18076570]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Hakoshima, T.]]
[[Category: Kitano, K.]]
[[Category: Maesaki, R.]]
[[Category: Takai, Y.]]
[[Category: Terawaki, S.]]
[[Category: cell adhesion]]
[[Category: protein-peptide complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:42:13 2008''