View source for 2iwt

[[Image:2iwt.gif|left|200px]]<br />
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'''THIOREDOXIN H2 (HVTRXH2) IN A MIXED DISULFIDE COMPLEX WITH THE TARGET PROTEIN BASI'''<br />

==Overview==
Thioredoxin is ubiquitous and regulates various target proteins through, disulfide bond reduction. We report the structure of thioredoxin (HvTrxh2, from barley) in a reaction intermediate complex with a protein substrate, barley alpha-amylase/subtilisin inhibitor (BASI). The crystal structure of, this mixed disulfide shows a conserved hydrophobic motif in thioredoxin, interacting with a sequence of residues from BASI through van der Waals, contacts and backbone-backbone hydrogen bonds. The observed structural, complementarity suggests that the recognition of features around protein, disulfides plays a major role in the specificity and protein disulfide, reductase activity of thioredoxin. This novel insight into the function of, thioredoxin constitutes a basis for comprehensive understanding of its, biological role. Moreover, comparison with structurally related proteins, shows that thioredoxin shares a mechanism with glutaredoxin and, glutathione transferase for correctly positioning substrate cysteine, residues at the catalytic groups but possesses a unique structural element, that allows recognition of protein disulfides.

==About this Structure==
2IWT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare] with FLC as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA]. 

==Reference==
Structural basis for target protein recognition by the protein disulfide reductase thioredoxin., Maeda K, Hagglund P, Finnie C, Svensson B, Henriksen A, Structure. 2006 Nov;14(11):1701-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17098195 17098195]
[[Category: Hordeum vulgare]]
[[Category: Protein complex]]
[[Category: Finnie, C.]]
[[Category: Hagglund, P.]]
[[Category: Henriksen, A.]]
[[Category: Maeda, K.]]
[[Category: Svensson, B.]]
[[Category: FLC]]
[[Category: alpha-amylase inhibitor]]
[[Category: amy2]]
[[Category: basi]]
[[Category: disulfide intermediate]]
[[Category: disulfide reductase]]
[[Category: oxidoreductase]]
[[Category: protease inhibitor]]
[[Category: redox]]
[[Category: serine protease inhibitor]]
[[Category: substrate recognition]]
[[Category: thioredoxin]]

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