View source for 1zux

[[Image:1zux.gif|left|200px]]

 {{Structure
|PDB= 1zux |SIZE=350|CAPTION= <scene name='initialview01'>1zux</scene>, resolution 1.850&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
}}

'''EosFP Fluorescent Protein- Green Form'''


==Overview==
Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.

==About this Structure==
1ZUX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUX OCA]. 

==Reference==
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP., Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15964985 15964985]
[[Category: Lobophyllia hemprichii]]
[[Category: Single protein]]
[[Category: Nar, H.]]
[[Category: Nienhaus, G U.]]
[[Category: Nienhaus, K.]]
[[Category: Wiedenmann, J.]]
[[Category: beta-elimination]]
[[Category: fluorescent protein]]
[[Category: peptide bond cleavage]]
[[Category: photoconversion]]

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