View source for 1yj5

[[Image:1yj5.gif|left|200px]]

 {{Structure
|PDB= 1yj5 |SIZE=350|CAPTION= <scene name='initialview01'>1yj5</scene>, resolution 2.80&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] 
|GENE= 
}}

'''Molecular architecture of mammalian polynucleotide kinase, a DNA repair enzyme'''


==Overview==
Mammalian polynucleotide kinase (PNK) is a key component of both the base excision repair (BER) and nonhomologous end-joining (NHEJ) DNA repair pathways. PNK acts as a 5'-kinase/3'-phosphatase to create 5'-phosphate/3'-hydroxyl termini, which are a necessary prerequisite for ligation during repair. PNK is recruited to repair complexes through interactions between its N-terminal FHA domain and phosphorylated components of either pathway. Here, we describe the crystal structure of intact mammalian PNK and a structure of the PNK FHA bound to a cognate phosphopeptide. The kinase domain has a broad substrate binding pocket, which preferentially recognizes double-stranded substrates with recessed 5' termini. In contrast, the phosphatase domain efficiently dephosphorylates single-stranded 3'-phospho termini as well as double-stranded substrates. The FHA domain is linked to the kinase/phosphatase catalytic domain by a flexible tether, and it exhibits a mode of target selection based on electrostatic complementarity between the binding surface and the phosphothreonine peptide.

==About this Structure==
1YJ5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJ5 OCA]. 

==Reference==
The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase., Bernstein NK, Williams RS, Rakovszky ML, Cui D, Green R, Karimi-Busheri F, Mani RS, Galicia S, Koch CA, Cass CE, Durocher D, Weinfeld M, Glover JN, Mol Cell. 2005 Mar 4;17(5):657-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15749016 15749016]
[[Category: Mus musculus]]
[[Category: Polynucleotide 5'-hydroxy-kinase]]
[[Category: Protein complex]]
[[Category: Bernstein, N K.]]
[[Category: Cass, C E.]]
[[Category: Cui, D.]]
[[Category: Durocher, D.]]
[[Category: Galicia, S.]]
[[Category: Glover, J N.M.]]
[[Category: Green, R.]]
[[Category: Karimi-Busheri, F.]]
[[Category: Koch, C A.]]
[[Category: Mani, R S.]]
[[Category: Rakovszky, M L.]]
[[Category: Weinfeld, M.]]
[[Category: Williams, R S.]]
[[Category: SO4]]
[[Category: beta sandwich]]
[[Category: p-loop]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:23:33 2008''