View source for 1wo3

[[Image:1wo3.gif|left|200px]]

 {{Structure
|PDB= 1wo3 |SIZE=350|CAPTION= <scene name='initialview01'>1wo3</scene>
|SITE= 
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] 
|GENE= 
}}

'''Solution structure of Minimal Mutant 1 (MM1): Multiple alanine mutant of non-native CHANCE domain'''


==Overview==
Zinc binding motifs have received much attention in the area of protein design. Here, we have tested the suitability of a recently discovered nonnative zinc binding structure as a protein design scaffold. A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in approximately 50% of the sequence. We next attempted to transplant binding faces from chosen bimolecular interactions onto one of these mutants, and many of the resulting "chimeras" were shown to adopt a native-like fold. These results both highlight the robust nature of small zinc binding domains and underscore the complexity of designing functional proteins, even using such small, highly ordered scaffolds as templates.

==Disease==
Known diseases associated with this structure: Blue-cone monochromacy OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=303900 303900]], Colorblindness, protan OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=303900 303900]], Rubenstein-Taybi syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600140 600140]]

==About this Structure==
1WO3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WO3 OCA]. 

==Reference==
Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting., Sharpe BK, Liew CK, Kwan AH, Wilce JA, Crossley M, Matthews JM, Mackay JP, Structure. 2005 Feb;13(2):257-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15698569 15698569]
[[Category: Histone acetyltransferase]]
[[Category: Single protein]]
[[Category: Crossley, M.]]
[[Category: Liew, C K.]]
[[Category: Mackay, J P.]]
[[Category: Matthews, J M.]]
[[Category: Sharpe, B K.]]
[[Category: Wilce, J A.]]
[[Category: ZN]]
[[Category: protein design]]
[[Category: zinc finger]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:59:29 2008''