View source for 1vfv

[[Image:1vfv.gif|left|200px]]

 {{Structure
|PDB= 1vfv |SIZE=350|CAPTION= <scene name='initialview01'>1vfv</scene>, resolution 1.85&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER'>ANP</scene>
|ACTIVITY= 
|GENE= 
}}

'''Crystal Structure of the Kif1A Motor Domain Complexed With Mg-AMPPNP'''


==Overview==
The motor protein kinesin moves along microtubules, driven by adenosine triphosphate (ATP) hydrolysis. However, it remains unclear how kinesin converts the chemical energy into mechanical movement. We report crystal structures of monomeric kinesin KIF1A with three transition-state analogs: adenylyl imidodiphosphate (AMP-PNP), adenosine diphosphate (ADP)-vanadate, and ADP-AlFx (aluminofluoride complexes). These structures, together with known structures of the ADP-bound state and the adenylyl-(beta,gamma-methylene) diphosphate (AMP-PCP)-bound state, show that kinesin uses two microtubule-binding loops in an alternating manner to change its interaction with microtubules during the ATP hydrolysis cycle; loop L11 is extended in the AMP-PNP structure, whereas loop L12 is extended in the ADP structure. ADP-vanadate displays an intermediate structure in which a conformational change in two switch regions causes both loops to be raised from the microtubule, thus actively detaching kinesin.

==About this Structure==
1VFV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VFV OCA]. 

==Reference==
KIF1A alternately uses two loops to bind microtubules., Nitta R, Kikkawa M, Okada Y, Hirokawa N, Science. 2004 Jul 30;305(5684):678-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15286375 15286375]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Hirokawa, N.]]
[[Category: Kikkawa, M.]]
[[Category: Nitta, R.]]
[[Category: Okada, Y.]]
[[Category: ANP]]
[[Category: MG]]
[[Category: kinesin]]
[[Category: microtubule]]
[[Category: motor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:45:05 2008''