View source for 2amn

==Solution structure of Fowlicidin-1, a novel Cathelicidin antimicrobial peptide from chicken==
<StructureSection load='2amn' size='340' side='right' caption='[[2amn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2amn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AMN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AMN FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2amn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2amn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2amn RCSB], [http://www.ebi.ac.uk/pdbsum/2amn PDBsum]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Cationic antimicrobial peptides are naturally occurring antibiotics that are actively being explored as a new class of anti-infective agents. We recently identified three cathelicidin antimicrobial peptides from chicken, which have potent and broad-spectrum antibacterial activities in vitro (Xiao Y, Cai Y, Bommineni YR, Fernando SC, Prakash O, Gilliland SE &amp; Zhang G (2006) J Biol Chem281, 2858-2867). Here we report that fowlicidin-1 mainly adopts an alpha-helical conformation with a slight kink induced by glycine close to the center, in addition to a short flexible unstructured region near the N terminus. To gain further insight into the structural requirements for function, a series of truncation and substitution mutants of fowlicidin-1 were synthesized and tested separately for their antibacterial, cytolytic and lipopolysaccharide (LPS)-binding activities. The short C-terminal helical segment after the kink, consisting of a stretch of eight amino acids (residues 16-23), was shown to be critically involved in all three functions, suggesting that this region may be required for the peptide to interact with LPS and lipid membranes and to permeabilize both prokaryotic and eukaryotic cells. We also identified a second segment, comprising three amino acids (residues 5-7) in the N-terminal flexible region, that participates in LPS binding and cytotoxicity but is less important in bacterial killing. The fowlicidin-1 analog, with deletion of the second N-terminal segment (residues 5-7), was found to retain substantial antibacterial potency with a significant reduction in cytotoxicity. Such a peptide analog may have considerable potential for development as an anti-infective agent.

Structure-activity relationships of fowlicidin-1, a cathelicidin antimicrobial peptide in chicken.,Xiao Y, Dai H, Bommineni YR, Soulages JL, Gong YX, Prakash O, Zhang G FEBS J. 2006 Jun;273(12):2581-93. PMID:16817888<ref>PMID:16817888</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Bommineni, Y R]]
[[Category: Dai, H]]
[[Category: Prakash, O]]
[[Category: Xiao, Y]]
[[Category: Zhang, G]]
[[Category: Antimicrobial protein]]
[[Category: Linear helix]]