View source for 4fs6

==Crystal structure of the Z-DNA hexamer CGCGCG at 500 mM CaCl2==
<StructureSection load='4fs6' size='340' side='right' caption='[[4fs6]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4fs6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FS6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FS6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fs5|4fs5]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fs6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fs6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fs6 RCSB], [http://www.ebi.ac.uk/pdbsum/4fs6 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500mM) using a temperature control technique, and their crystal structures were determined at 1.3A resolution. Mg2+ and Ca2+ cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes; however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes through coordination bonds of PO...(Mg2+ or Ca2+)...OP. A symmetrical hexagonal Z-DNA duplex assembly model may explain DNA condensation caused by alkaline earth salts. These structures offer insights into the functions of alkaline earth cations essential to the structures and assembly of Z-DNA duplexes.

Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl and CaCl,Chatake T, Sunami T J Inorg Biochem. 2013 Mar 19;124C:15-25. doi: 10.1016/j.jinorgbio.2013.03.004. PMID:23578858<ref>PMID:23578858</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
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</StructureSection>
[[Category: Chatake, T]]
[[Category: Sunami, T]]
[[Category: Ca2+ binding]]
[[Category: Dna]]
[[Category: Z-dna]]