View source for 3ax1

==Molecular insights into miRNA processing by Arabidopsis Serrate==
<StructureSection load='3ax1' size='340' side='right' caption='[[3ax1]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ax1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AX1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SE, At2g27100, T20P8.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ax1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ax1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ax1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ax1 PDBsum]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
In plant, primary transcripts (pri-miRNAs) transcribed from miRNA genes by RNA polymerase II are first processed into stem-loop pre-miRNAs and further chopped into approximately 21 nt long miRNAs by RNase III-like enzyme DCL1. SERRATE (SE) protein is an essential component for miRNA processing by assisting DCL1 for accurate cleavage. Here we report the crystal structure of Arabidopsis SE core (residues 194-543) at 2.7 A. SE core adopts the 'walking man-like' topology with N-terminal alpha helices, C-terminal non-canonical zinc-finger domain and novel Middle domain resembling the leading leg, the lagging leg and the body, respectively. Pull-down assay shows that SE core provides the platform for HYL1 and DCL1 binding, whereas in vitro RNA binding and in vivo mutant rescue experiments suggest that the non-canonical zinc-finger domain coupled with C-terminal tail binds miRNA precursors. SE presumably works as a scaffold-like protein capable of binding both protein and RNA to guide the positioning of miRNA precursor toward DCL1 catalytic site within miRNA processing machinery in plant.

Molecular insights into miRNA processing by Arabidopsis thaliana SERRATE.,Machida S, Chen HY, Adam Yuan Y Nucleic Acids Res. 2011 Jun 17. PMID:21685453<ref>PMID:21685453</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Chen, H Y]]
[[Category: Machida, S]]
[[Category: Yuan, Y A]]
[[Category: Mirna processing]]
[[Category: Protein binding]]
[[Category: Serrate]]