View source for 1ppg

[[Image:1ppg.gif|left|200px]]

 {{Structure
|PDB= 1ppg |SIZE=350|CAPTION= <scene name='initialview01'>1ppg</scene>, resolution 2.3&Aring;
|SITE= 
|LIGAND= 
|ACTIVITY= [http://en.wikipedia.org/wiki/Leukocyte_elastase Leukocyte elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.37 3.4.21.37] 
|GENE= 
}}

'''THE REFINED 2.3 ANGSTROMS CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE ELASTASE IN A COMPLEX WITH A VALINE CHLOROMETHYL KETONE INHIBITOR'''


==Overview==
The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A). The enzyme structure is very similar to that recently observed in a complex formed with the ovomucoid third domain from turkey [(1986) EMBO J. 5,2453-2458]. The rms deviation of all alpha-carbon atoms is 0.32 A. The peptidic inhibitor is bound in a similar overall conformation as the ovomucoid binding segment. Covalent bonds are formed between Val-P1 of the inhibitor and His-57 NE2 and Ser-195 OG of the enzyme. The carbonyl carbon is tetrahedrally deformed to a hemiketal. The valine side chain is arranged in the S1 pocket in the g-conformation.

==Disease==
Known diseases associated with this structure: Hematopoiesis, cyclic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=130130 130130]], Neutropenia, congenital OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=130130 130130]]

==About this Structure==
1PPG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PPG OCA]. 

==Reference==
The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor., Wei AZ, Mayr I, Bode W, FEBS Lett. 1988 Jul 18;234(2):367-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3391280 3391280]
[[Category: Leukocyte elastase]]
[[Category: Single protein]]
[[Category: Bode, W.]]
[[Category: Wei, A-Z.]]
[[Category: hydrolase(serine proteinase)]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:27:18 2008''