View source for Cas9

[[Cas9]] is the  RNA-guided [[DNA]] [[endonuclease]] used by the CRISPR(clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.

The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences. 

[[Image:Layout for schematic and structure with structure.png]]

Articles in Proteopedia concerning Cas9 include:
* any?

==3D structures of Cas9==

===''Streptococcus pyogenes'' Cas9===

* [[4un3]], [[4un4]], and [[4un5]] -  ''S. pyogenes'' Cas9 bound to sgRNA and target DNA
[[Image:4un3 labeled.png|right]]
* [[4oo8]] -  ''S. pyogenes'' Cas9 bound to sgRNA and target DNA
* [[4cmp]]
* [[4cmq]]  - Mn<sup>2+</sup>-bound ''S. pyogenes'' Cas9

=== ''Actinomyces naeslundii'' Cas9=== 
*[[4oge]]
*[[4ogc]]  - Mn<sup>2+</sup>-bound ''A.s naeslundii'' Cas9

==See Also==
[[HNH endonuclease]] <br/>



== References ==
<references/>

[[Category: Crispr]]
[[Category: Crispr-associated]]
[[Category: endonuclease]]