View source for 1kew

[[Image:1kew.jpg|left|200px]]

 {{Structure
|PDB= 1kew |SIZE=350|CAPTION= <scene name='initialview01'>1kew</scene>, resolution 1.80&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=TYD:THYMIDINE-5'-+DIPHOSPHATE'>TYD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/dTDP-glucose_4,6-dehydratase dTDP-glucose 4,6-dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.46 4.2.1.46] 
|GENE= rmlB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 Salmonella enterica subsp. enterica serovar Typhimurium])
}}

'''The crystal structure of dTDP-D-glucose 4,6-dehydratase (RmlB) from Salmonella enterica serovar Typhimurium with thymidine diphosphate bound'''


==Overview==
dTDP-D-glucose 4,6-dehydratase (RmlB) was first identified in the L-rhamnose biosynthetic pathway, where it catalyzes the conversion of dTDP-D-glucose into dTDP-4-keto-6-deoxy-D-glucose. The structures of RmlB from Salmonella enterica serovar Typhimurium in complex with substrate deoxythymidine 5'-diphospho-D-glucose (dTDP-D-glucose) and deoxythymidine 5'-diphosphate (dTDP), and RmlB from Streptococcus suis serotype 2 in complex with dTDP-D-glucose, dTDP, and deoxythymidine 5'-diphospho-D-pyrano-xylose (dTDP-xylose) have all been solved at resolutions between 1.8 A and 2.4 A. The structures show that the active sites are highly conserved. Importantly, the structures show that the active site tyrosine functions directly as the active site base, and an aspartic and glutamic acid pairing accomplishes the dehydration step of the enzyme mechanism. We conclude that the substrate is required to move within the active site to complete the catalytic cycle and that this movement is driven by the elimination of water. The results provide insight into members of the SDR superfamily.

==About this Structure==
1KEW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEW OCA]. 

==Reference==
Toward a structural understanding of the dehydratase mechanism., Allard ST, Beis K, Giraud MF, Hegeman AD, Gross JW, Wilmouth RC, Whitfield C, Graninger M, Messner P, Allen AG, Maskell DJ, Naismith JH, Structure. 2002 Jan;10(1):81-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11796113 11796113]
[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
[[Category: Single protein]]
[[Category: dTDP-glucose 4,6-dehydratase]]
[[Category: Allard, S T.M.]]
[[Category: Allen, A G.]]
[[Category: Beis, K.]]
[[Category: Giraud, M F.]]
[[Category: Graninger, M.]]
[[Category: Gross, J W.]]
[[Category: Hegeman, A D.]]
[[Category: Messner, P.]]
[[Category: Naismith, J H.]]
[[Category: Whitfield, C.]]
[[Category: GOL]]
[[Category: NAD]]
[[Category: TYD]]
[[Category: rossmann fold]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:32 2008''