View source for 1jw2

[[Image:1jw2.gif|left|200px]]

 {{Structure
|PDB= 1jw2 |SIZE=350|CAPTION= <scene name='initialview01'>1jw2</scene>
|SITE= 
|LIGAND= 
|ACTIVITY= 
|GENE= 
}}

'''SOLUTION STRUCTURE OF HEMOLYSIN EXPRESSION MODULATING PROTEIN Hha FROM ESCHERICHIA COLI. Ontario Centre for Structural Proteomics target EC0308_1_72; Northeast Structural Genomics Target ET88'''


==Overview==
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

==About this Structure==
1JW2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JW2 OCA]. 

==Reference==
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11854485 11854485]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Arrowsmith, C H.]]
[[Category: Chang, X.]]
[[Category: Edwards, A M.]]
[[Category: NESG, Northeast Structural Genomics Consortium.]]
[[Category: Savchenko, A.]]
[[Category: Yee, A.]]
[[Category: hemolysin expression modulating protein]]
[[Category: hha]]
[[Category: nesg]]
[[Category: northeast structural genomics consortium]]
[[Category: ocsp]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomic]]

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