View source for 1jl3

[[Image:1jl3.jpg|left|200px]]

 {{Structure
|PDB= 1jl3 |SIZE=350|CAPTION= <scene name='initialview01'>1jl3</scene>, resolution 1.60&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
|ACTIVITY= 
|GENE= arsC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
}}

'''Crystal Structure of B. subtilis ArsC'''


==Overview==
Arsenate is an abundant oxyanion that, because of its ability to mimic the phosphate group, is toxic to cells. Arsenate reductase (EC; encoded by the arsC gene in bacteria) participates to achieve arsenate resistance in both prokaryotes and yeast by reducing arsenate to arsenite; the arsenite is then exported by a specific transporter. The crystal structure of Bacillus subtilis arsenate reductase in the reduced form with a bound sulfate ion in its active site is solved at 1.6-A resolution. Significant structural similarity is seen between arsenate reductase and bovine low molecular weight protein tyrosine phosphatase, despite very low sequence identity. The similarity is especially high between their active sites. It is further confirmed that this structural homology is relevant functionally by showing the phosphatase activity of the arsenate reductase in vitro. Thus, we can understand the arsenate reduction in the light of low molecular weight protein tyrosine phosphatase mechanism and also explain the catalytic roles of essential residues such as Cys-10, Cys-82, Cys-89, Arg-16, and Asp-105. A "triple cysteine redox relay" is proposed for the arsenate reduction mechanism.

==About this Structure==
1JL3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL3 OCA]. 

==Reference==
Bacillus subtilis arsenate reductase is structurally and functionally similar to low molecular weight protein tyrosine phosphatases., Bennett MS, Guan Z, Laurberg M, Su XD, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13577-82. Epub 2001 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11698660 11698660]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Bennett, M S.]]
[[Category: Su, X D.]]
[[Category: SO4]]
[[Category: alpha-beta fold]]
[[Category: arsenate reductase]]
[[Category: ptp-loop]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:04:55 2008''