View source for 1ih7

[[Image:1ih7.jpg|left|200px]]

 {{Structure
|PDB= 1ih7 |SIZE=350|CAPTION= <scene name='initialview01'>1ih7</scene>, resolution 2.21&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> and <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] 
|GENE= gp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10692 Enterobacteria phage RB18])
}}

'''High-Resolution Structure of Apo RB69 DNA Polymerase'''


==Overview==
We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.

==About this Structure==
1IH7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IH7 OCA]. 

==Reference==
Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11389835 11389835]
[[Category: DNA-directed DNA polymerase]]
[[Category: Enterobacteria phage rb18]]
[[Category: Single protein]]
[[Category: Franklin, M C.]]
[[Category: Steitz, T A.]]
[[Category: Wang, J.]]
[[Category: GMP]]
[[Category: K]]
[[Category: dna polymerase]]
[[Category: finger]]
[[Category: palm]]
[[Category: thumb]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:50:18 2008''