View source for 1yla

==Ubiquitin-conjugating enzyme E2-25 kDa (Huntington interacting protein 2)==
<StructureSection load='1yla' size='340' side='right' caption='[[1yla]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1yla]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YLA FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HIP2, LIG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yla RCSB], [http://www.ebi.ac.uk/pdbsum/1yla PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yl/1yla_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
E2-25K/Hip2 is an unusual ubiquitin-conjugating enzyme that interacts with the frameshift mutant of ubiquitin B (UBB+1) and has been identified as a crucial factor regulating amyloid-beta neurotoxicity. To study the structural basis of the neurotoxicity mediated by the E2-25K/UBB+1 interaction, we determined the three-dimensional structures of UBB+1, E2 25K and the E2-25K/Ubiquitin and E2-25K/UBB+1 complexes. The structures revealed that ubiquitin or UBB+1 is bound to E2-25K via the enzyme's MGF motif and residues in alpha 9 of the enzyme. Polyubiquitylation assays, together with analyses of various E2-25K mutants, showed that disrupting UBB+1 binding markedly diminishes synthesis of neurotoxic UBB+1 anchored polyUb. These results suggest that the interaction between E2-25K and UBB+1 is critical for the synthesis and accumulation of UBB+1-anchored polyUb, which results in proteasomal inhibition and neuronal cell death.

Structural basis of E2-25K/UBB+1 interaction leading to proteasome inhibition and neurotoxicity.,Ko S, Kang GB, Song SM, Lee JG, Shin DY, Yoon J, Sheng Y, Cheong C, Jeon YH, Jung YK, Arrowsmith CH, Avvakumov GV, Dhe-Paganon S, Yoo YJ, Eom SH, Lee W J Biol Chem. 2010 Sep 8. PMID:20826778<ref>PMID:20826778</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Ubiquitin conjugating enzyme|Ubiquitin conjugating enzyme]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Ubiquitin--protein ligase]]
[[Category: Arrowsmith, C.]]
[[Category: Avvakumov, G V.]]
[[Category: Bochkarev, A.]]
[[Category: Choe, J.]]
[[Category: Dhe-paganon, S.]]
[[Category: Edwards, A.]]
[[Category: Kozieradzki, I.]]
[[Category: Mackenzie, F.]]
[[Category: Newman, E M.]]
[[Category: SGC, Structural Genomics Consortium.]]
[[Category: Sundstrom, M.]]
[[Category: Ligase]]
[[Category: Sgc]]
[[Category: Structural genomic]]
[[Category: Structural genomics consortium]]
[[Category: Ubiquitin]]
[[Category: Ubiquitin- conjugating enzyme]]