View source for 2bg8

[[Image:2bg8.gif|left|200px]]<br />
<applet load="2bg8" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2bg8, resolution 2.5&Aring;" />
'''BACILLUS CEREUS METALLO-BETA-LACTAMASE (BCII) ARG (121) CYS MUTANT. SOLVED AT PH4.5 USING 20 MICROMOLAR ZNSO4 IN THE BUFFER. 1MM DTT AND 1MM TCEP-HCL WERE USED AS REDUCING AGENTS.'''<br />

==Overview==
The zinc-dependent metallo-beta-lactamases are a group of bacterial, enzymes that pose a threat to the future efficacy of present-day, antibiotics. Their mechanism is poorly understood, and there are no, clinically useful inhibitors. While most members of the group contain two, tightly bound zinc ions in their active sites, the Bacillus cereus enzyme, has a much lower affinity for its second zinc (Zn2), thought to be due to, the presence of Arg121 immediately beneath the floor of the active site, (cf. Cys/Ser/His121 in the bizinc enzymes). Crystal structures of the, Arg121Cys mutant of the B. cereus 569/H/9 enzyme were solved at pH 7.0, 5.0, and 4.5, each in the presence of either 20 microM or 20 mM Zn(2+) to, generate the mono- and bizinc forms, respectively. Surprisingly, the, structure ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15779910 (full description)]]

==About this Structure==
2BG8 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]] with ZN, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BG8 OCA]]. 

==Reference==
Effect of pH on the active site of an Arg121Cys mutant of the metallo-beta-lactamase from Bacillus cereus: implications for the enzyme mechanism., Davies AM, Rasia RM, Vila AJ, Sutton BJ, Fabiane SM, Biochemistry. 2005 Mar 29;44(12):4841-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15779910 15779910]
[[Category: Bacillus cereus]]
[[Category: Protein complex]]
[[Category: Davies, A.M.]]
[[Category: Fabiane, S.M.]]
[[Category: Rasia, R.M.]]
[[Category: Sutton, B.J.]]
[[Category: Vila, A.J.]]
[[Category: GOL]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: antibiotic resistance]]
[[Category: hydrolase]]
[[Category: metallo-beta-lactamase]]
[[Category: zinc]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 16:45:17 2007''