View source for 1gwr

[[Image:1gwr.gif|left|200px]]

 {{Structure
|PDB= 1gwr |SIZE=350|CAPTION= <scene name='initialview01'>1gwr</scene>, resolution 2.4&Aring;
|SITE= <scene name='pdbsite=ESA:Est+Binding+Site+For+Chain+B'>ESA</scene>
|LIGAND= <scene name='pdbligand=EST:ESTRADIOL'>EST</scene>
|ACTIVITY= 
|GENE= 
}}

'''HUMAN OESTROGEN RECEPTOR ALPHA LIGAND-BINDING DOMAIN IN COMPLEX WITH 17BETA-OESTRADIOL AND TIF2 NRBOX3 PEPTIDE'''


==Overview==
The activation function 2/ligand-dependent interaction between nuclear receptors and their coregulators is mediated by a short consensus motif, the so-called nuclear receptor (NR) box. Nuclear receptors exhibit distinct preferences for such motifs depending both on the bound ligand and on the NR box sequence. To better understand the structural basis of motif recognition, we characterized the interaction between estrogen receptor alpha and the NR box regions of the p160 coactivator TIF2. We have determined the crystal structures of complexes between the ligand-binding domain of estrogen receptor alpha and 12-mer peptides from the Box B2 and Box B3 regions of TIF2. Surprisingly, the Box B3 module displays an unexpected binding mode that is distinct from the canonical LXXLL interaction observed in other ligand-binding domain/NR box crystal structures. The peptide is shifted along the coactivator binding site in such a way that the interaction motif becomes LXXYL rather than the classical LXXLL. However, analysis of the binding properties of wild type NR box peptides, as well as mutant peptides designed to probe the Box B3 orientation, suggests that the Box B3 peptide primarily adopts the "classical" LXXLL orientation in solution. These results highlight the potential difficulties in interpretation of protein-protein interactions based on co-crystal structures using short peptide motifs.

==Disease==
Known diseases associated with this structure: Atherosclerosis, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=133430 133430]], Breast cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=133430 133430]], Estrogen resistance OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=133430 133430]], HDL response to hormone replacement, augmented OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=133430 133430]], Migraine, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=133430 133430]], Myocardial infarction, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=133430 133430]]

==About this Structure==
1GWR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWR OCA]. 

==Reference==
Interaction of transcriptional intermediary factor 2 nuclear receptor box peptides with the coactivator binding site of estrogen receptor alpha., Warnmark A, Treuter E, Gustafsson JA, Hubbard RE, Brzozowski AM, Pike AC, J Biol Chem. 2002 Jun 14;277(24):21862-8. Epub 2002 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11937504 11937504]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Brzozowski, A M.]]
[[Category: Pike, A C.W.]]
[[Category: EST]]
[[Category: activator]]
[[Category: af2 coactivator]]
[[Category: agonist]]
[[Category: alternative splicin]]
[[Category: dna-binding]]
[[Category: nuclear protein]]
[[Category: nuclear receptor]]
[[Category: phosphorylation]]
[[Category: polymorphism]]
[[Category: receptor]]
[[Category: ster binding]]
[[Category: transactivation]]
[[Category: transcripti regulation]]
[[Category: transcription factor]]
[[Category: zinc finger]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:28:59 2008''