View source for 1guo

[[Image:1guo.gif|left|200px]]

 {{Structure
|PDB= 1guo |SIZE=350|CAPTION= <scene name='initialview01'>1guo</scene>, resolution 2.50&Aring;
|SITE= <scene name='pdbsite=T11:Type+2+Binding+Site'>T11</scene>
|LIGAND= <scene name='pdbligand=MOO:MOLYBDATE ION'>MOO</scene>
|ACTIVITY= 
|GENE= 
}}

'''MOPII FROM CLOSTRIDIUM PASTEURIANUM COMPLEXED WITH MOLYBDATE'''


==Overview==
MopII from Clostridium pasteurianum is a molbindin family member. These proteins may serve as intracellular storage facilities for molybdate, which they bind with high specificity. High resolution structures of MopII in a number of states, including the first structure of an apo-molbindin, together with calorimetric data, allow us to describe ligand binding and provide support for the proposed storage function of the protein. MopII assembles as a trimer of dimers and binds eight oxyanions at two types of binding sites located at intersubunit interfaces. Two type 1 sites are on the molecular 3-fold axis and three pairs of type 2 sites occur on the molecular 2-fold axes. The hexamer is largely unaffected by the binding of ligand. Molybdate is admitted to the otherwise inaccessible type 2 binding sites by the movement of the N-terminal residues of each protein chain. This contrasts with the structurally related molybdate-dependent transcriptional regulator ModE, which undergoes extensive conformational rearrangements on ligand binding. Despite similarities between the binding sites of ModE and the type 2 sites of MopII the molbindin has a significantly reduced ligand affinity, due, in part, to the high density of negative charges at the center of the hexamer. In the absence of ligand this effects the movement of an important lysine side chain, thereby partially inactivating the binding sites. The differences are consistent with a biological role in molybdate storage/buffering.

==About this Structure==
1GUO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUO OCA]. 

==Reference==
Passive acquisition of ligand by the MopII molbindin from Clostridium pasteurianum: structures of apo and oxyanion-bound forms., Schuttelkopf AW, Harrison JA, Boxer DH, Hunter WN, J Biol Chem. 2002 Apr 26;277(17):15013-20. Epub 2002 Feb 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11836258 11836258]
[[Category: Clostridium pasteurianum]]
[[Category: Single protein]]
[[Category: Harrison, J A.]]
[[Category: Hunter, W N.]]
[[Category: Schuettelkopf, A W.]]
[[Category: MOO]]
[[Category: molbindin]]
[[Category: molybdate binding]]
[[Category: molybdenum]]
[[Category: mop]]
[[Category: multigene fam]]

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